Author, as appears in the article.: Nadal, L.; Garcia, N.; Hurtado, E.; Simó, A.; Tomàs, M.; Lanuza, M.A.; Cilleros, V.; Tomàs, J.

Department: Ciències Mèdiques Bàsiques

URV's Author/s: NADAL MAGRIÑÀ, LAURA; Garcia, N.; HURTADO CABALLERO, ERICA; SIMÓ OLLÉ, ANNA; TOMAS MARGINET, MARTA; LANUZA ESCOLANO, MARÍA ANGEL; CILLEROS MAÑÉ, VÍCTOR; Tomàs, J.

Keywords: cholinergic synapses motor nerve terminal Neuromuscular junction

Abstract: The development of the nervous system involves the overproduction of synapses but connectivity is refined by Hebbian activity-dependent axonal competition. The newborn skeletal muscle fibers are polyinnervated but, at the end of the competition process, some days later, become innervated by a single axon. We used quantitative confocal imaging of the autofluorescent axons from transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice to investigate the possible cooperation of the muscarinic autoreceptors (mAChR, M1-, M2- and M4-subtypes) and the tyrosine kinase B (TrkB) receptor in the control of axonal elimination after the mice Levator auris longus (LAL) muscle had been exposed to several selective antagonist of the corresponding receptor pathways in vivo. Our previous results show that M1, M2 and TrkB signaling individually increase axonal loss rate around P9. Here we show that although the M1 and TrkB receptors cooperate and add their respective individual effects to increase axonal elimination rate even more, the effect of the M2 receptor is largely independent of both M1 and TrkB receptors. Thus both, cooperative and non-cooperative signaling mechanisms contribute to developmental synapse elimination.

Research group: Unitat d'Histologia i Neurobiologia

Thematic Areas: Ciències de la salut Ciencias de la salud Health sciences

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1663-4365

Author identifier: N/D; N/D; N/D; N/D; N/D; N/D; N/D; N/D

Record's date: 2017-03-21

Journal volume: 9

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.frontiersin.org/articles/10.3389/fnagi.2017.00024/full

Article's DOI: 10.3389/fnagi.2017.00024

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

First page: Art.num. 24

Publication Type: Article Artículo Article