Author, as appears in the article.: Mas, A.; González, B.; Beltran, G.; Cullen, P.J.; Torija, M.J.

Department: Bioquímica i Biotecnologia

URV's Author/s: MAS BARON, ALBERTO; González, B.; BELTRAN CASELLAS, GEMMA; Cullen, P.J.; TORIJA MARTÍNEZ, MARÍA JESÚS

Keywords: filamentous growth pseudohyphal growth quorum sensing

Abstract: In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in 61278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.

Research group: Biotecnologia Enològica

Thematic Areas: Enologia Enología Oenology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1664-042X

Author identifier: 0000-0002-0763-1679; n/a; 0000-0002-7071-205X; n/a; 0000-0001-6419-0745

Record's date: 2017-05-18

Journal volume: 8

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: http://journal.frontiersin.org/article/10.3389/fphys.2017.00148/full

Article's DOI: 10.3389/fphys.2017.00148

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

First page: Art.num. 148

Publication Type: Article Artículo Article