Author, as appears in the article.: Guerrini, L.; Pedrol, E.; Garcia-Algar, M.; Massons, J.; Nazarenus, M.; Martínez, J.; Rodenas, A.; Fernandez-Carrascal, A.; Aguiló, M.; Estevez, L.G.; Calvo, I.; Olano-Daza, A.; Garcia-Rico, E.; Díaz, F.; Alvarez-Puebla, R.A.

Department: Química Física i Inorgànica

URV's Author/s: GUERRINI , LUCA; Pedrol, E.; Garcia-Algar, M.; Massons, J.; Nazarenus, M.; Martínez, J.; RODENAS SEGUI, AIRAN; Fernandez-Carrascal, A.; AGUILÓ DÍAZ, MAGDALENA; Estevez, L.G.; Calvo, I.; Olano-Daza, A.; Garcia-Rico, E.; DÍAZ GONZÁLEZ, FRANCISCO MANUEL; ALVAREZ PUEBLA, RAMON ANGEL

Abstract: Metastatic cancer patients require a continuous monitoring during the sequential treatment cycles to carefully evaluate their disease evolution. Repetition of biopsies is very invasive and not always feasible. Herein, we design and demonstrate a 3D-flow focusing microfluidic device, where all optics are integrated into the chip, for the fluorescence quantification of CTCs in real samples. To test the chip performance, two cell membrane targets, the epithelial cell adhesion molecule, EpCAM, and the receptor tyrosine-protein kinase, HER2, are selected. The efficiency of the platform is demonstrated on cell lines and in a variety of healthy donors and metastatic-breast cancer patients.

Research group: Física i Cristal·lografia de Nanomaterials Grupo de Plasmonica y Ultradetección Física i Cristal.lografia de Materials

Thematic Areas: Chemistry Química Química

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 2045-2322

Author identifier: 0000-0002-2925-1562; ; ; ; ; ; ; ; 0000-0001-6130-9579; ; ; ; ; 0000-0003-4581-4967; 0000-0003-4770-5756

Record's date: 2017-10-20

Journal volume: 7

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.nature.com/articles/s41598-017-04033-9

Funding program: altres; ICREA academia award 2010; ICREA; ICREA-02 altres; Grups consolidats Generalitat de Catalunya; SGR; 2014SGR480 altres; Grups consolidats Generalitat de Catalunya; SGR; 2014SGR1358 plan; Ministerio de Economia y Competitividad; CTQ2014; CTQ2014-59808R plan; Ministerio de Economia y Competitividad; TEC; TEC2014- 55948-R plan; Ministerio de Economia y Competitividad; MAT; MAT2013-47395-C4-4-R

Article's DOI: 10.1038/s41598-017-04033-9

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

First page: 3677

Publication Type: Article Artículo Article