Author, as appears in the article.: Borrull, F. ; Baciu, T. ; Aguilar, M. C. ; Callul, M.

Department: Química Analítica i Química Orgànica

URV's Author/s: BORRULL BALLARÍN, FRANCESC; Baciu, T. ; AGUILAR ANGUERA, MARIA DEL CARMEN; CALULL BLANCH, MARTA

Keywords: chiral CE Cyclodextrin segmental hair analysis

Abstract: A suitable method has been developed and validated for simultaneously determining cocaine and its major metabolite, benzoylecgonine, 6-acetylmorphine, codeine, morphine and the methadone enantiomers in human hair samples by the in-line coupling between SPE and CyD-assisted CE with a previous sample pretreatment procedure based on pressurized liquid extraction. Optimal separation was achieved on a fused silica-capillary of 50 μm i.d. and 80 cm total length using 11 mM α-CyD in an aqueous solution of 80 mM sodium phosphate at pH 2.5 as the separation medium and an applied voltage of 30 kV. The SPE-CE device consisted of a short length of capillary packed with Oasis HLB sorbent, which was inserted near to the inlet end of the CE capillary. Several parameters affecting the in-line preconcentration were evaluated. The LOQs reached for hair samples were in the range of 0.3–2.5 ng/mg with satisfactory analytical precision in both intraday and day-to-day experiments (RSDs <13%). Relative recoveries greater than 80% were obtained. The method has successfully been applied to the determination of these drugs of abuse in segmented hair from drug abusers who were undergoing methadone maintenance treatment. The results were consistent with the patients' statements, indicating that the method established herein can be used for verifying a history of drug abuse.

Research group: Grup de Cromatografia. Aplicacions Mediambientals

Thematic Areas: Química Química Chemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0731-7085

Author identifier: 0000-0003-2718-9336; ; 0000-0002-5913-2599; 0000-0002-5258-1949

Record's date: 2018-03-06

Last page: 428

Journal volume: 131

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0731708516306057

Funding program: plan; CTQ2014-52617

Article's DOI: 10.1016/j.jpba.2016.09.017

Entity: Universitat Rovira i Virgili

Journal publication year: 2016

First page: 420

Publication Type: Article Artículo Article