Author, as appears in the article.: Aragonès G, Auguet T, Armengol S, Berlanga A, Guiu-Jurado E, Aguilar C, Martínez S, Sabench F, Porras JA, Ruiz MD, Hernández M, Sirvent JJ, Del Castillo D, Del Castillo D, Richart C.

Department: Ciències Mèdiques Bàsiques Medicina i Cirurgia Bioquímica i Biotecnologia

e-ISSN: 1422-0067

URV's Author/s: Aguilar Crespillo, Carmen Isabel / ARAGONÈS BARGALLÓ, GEMMA / ARMENGOL LÓPEZ, SANDRA / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Hernández González, Mercè / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Perefe

Keywords: Steatohepatitis no alcoholica Simple steatosis Pnpla3 Obesitat mòrbida Non-alcoholic steatohepatitis Non-alcoholic fatty liver disease Non alcoholic fat liver disease Morbid obesity Fatty acid metabolism pnpla3 non-alcoholic steatohepatitis non-alcoholic fatty liver disease morbid obesity fatty acid metabolism

Abstract: Recent reports suggest a role for the Patatin-like phospholipase domain-containing protein 3 (PNPLA3) in the pathology of non-alcoholic fatty liver disease (NAFLD). Lipid deposition in the liver seems to be a critical process in the pathogenesis of NAFLD. The aim of the present work was to evaluate the association between the liver PNPLA3 expression, key genes of lipid metabolism, and the presence of NAFLD in morbidly obese women. We used real-time polymerase chain reaction (PCR) analysis to analyze the hepatic expression of PNPLA3 and lipid metabolism-related genes in 55 morbidly obese subjects with normal liver histology (NL, n = 18), simple steatosis (SS, n = 20), and non-alcoholic steatohepatitis (NASH, n = 17). Liver biopsies were collected during bariatric surgery. We observed that liver PNPLA3 expression was increased in NAFLD than in NL. It was also upregulated in SS than in NL. Interestingly, we found that the expression of PNPLA3 was significantly higher in severe than mild SS group. In addition, the expression of the transcription factors LXRα, PPARα, and SREBP2 was positively correlated with PNPLA3 liver expression. Regarding rs738409 polymorphism, GG genotype was positive correlated with the presence of NASH. In conclusion, our results show that PNPLA3 could be related to lipid accumulation in liver, mainly in the development and progression of simple steatosis.

Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14220067

Author's mail: joseantonio.porras@urv.cat mariasalome.martinez@urv.cat cristobalmanuel.richart@urv.cat danieldel.castillo@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat fatima.sabench@urv.cat merce.hernandez@urv.cat

Author identifier: 0000-0001-6418-1822 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0456-3102 0000-0001-7024-7824 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X 0000-0002-9262-8756

Record's date: 2023-05-21

Journal volume: 17

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1422-0067/17/5/630

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Molecular Sciences. 17 (5): E630-

APA: Aragonès G, Auguet T, Armengol S, Berlanga A, Guiu-Jurado E, Aguilar C, Martínez S, Sabench F, Porras JA, Ruiz MD, Hernández M, Sirvent JJ, Del Castil (2016). PNPLA3 expression is related to liver steatosis in morbidly obese women with non-alcoholic fatty liver disease. International Journal Of Molecular Sciences, 17(5), E630-. DOI: 10.3390/ijms17050630

Article's DOI: 10.3390/ijms17050630

Entity: Universitat Rovira i Virgili

Journal publication year: 2016

Publication Type: Journal Publications