Author, as appears in the article.: Guiu-Jurado E., Auguet T., Berlanga A., Aragonès G., Aguilar C., Sabench F., Armengol S., Porras J., Martí A., Jorba R., Hernández M., del Castillo D., Richart C.

Department: Bioquímica i Biotecnologia Medicina i Cirurgia

e-ISSN: 1422-0067

URV's Author/s: Aguilar Crespillo, Carmen Isabel / ARAGONÈS BARGALLÓ, GEMMA / ARMENGOL LÓPEZ, SANDRA / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Hernández González, Mercè / Jorba Martin, Rosa Maria / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, F

Keywords: Moderate obesity Fatty acid metabolism De novo fatty acid synthesis Adipose tissue fatty acid metabolism de novo fatty acid synthesis adipose tissue

Abstract: The purpose of this work was to evaluate the expression of fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot to analyze visceral (VAT) and subcutaneous (SAT) adipose tissue mRNA expression involved in de novo fatty acid synthesis (ACC1, FAS), fatty acid oxidation (PPARα, PPARδ) and inflammation (IL6, TNFα), in normal weight control women (BMI < 25 kg/m2, n = 35) and moderately obese women (BMI 30-38 kg/m2, n = 55). In SAT, ACC1, FAS and PPARα mRNA expression were significantly decreased in moderately obese women compared to controls. The downregulation reported in SAT was more pronounced when BMI increased. In VAT, lipogenic-related genes and PPARα were similar in both groups. Only PPARδ gene expression was significantly increased in moderately obese women. As far as inflammation is concerned, TNFα and IL6 were significantly increased in moderate obesity in both tissues. Our results indicate that there is a progressive downregulation in lipogenesis in SAT as BMI increases, which suggests that SAT decreases the synthesis of fatty acid de novo during the development of obesity, whereas in VAT lipogenesis remains active regardless of the degree of obesity

Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14220067

Author's mail: joseantonio.porras@urv.cat rosamaria.jorba@urv.cat cristobalmanuel.richart@urv.cat danieldel.castillo@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat fatima.sabench@urv.cat merce.hernandez@urv.cat

Author identifier: 0000-0001-6418-1822 0000-0003-3307-4340 0000-0003-0852-6739 0000-0003-0456-3102 0000-0001-7024-7824 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X 0000-0002-9262-8756

Last page: 29922

Record's date: 2023-02-22

Journal volume: 16

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1422-0067/16/12/26206

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Molecular Sciences. 16 (12): 29911-29922

APA: Guiu-Jurado E., Auguet T., Berlanga A., Aragonès G., Aguilar C., Sabench F., Armengol S., Porras J., Martí A., Jorba R., Hernández M., del Castillo D. (2015). Downregulation of de novo fatty acid synthesis in subcutaneous adipose tissue of moderately obese women. International Journal Of Molecular Sciences, 16(12), 29911-29922. DOI: 10.3390/ijms161226206

Article's DOI: 10.3390/ijms161226206

Entity: Universitat Rovira i Virgili

Journal publication year: 2015

First page: 29911

Publication Type: Journal Publications