Author, as appears in the article.: Auguet T, Aragonès G, Berlanga A, Guiu-Jurado E, Martí A, Martínez S, Sabench F, Hernández M, Aguilar C, Sirvent JJ, Del Castillo D, Del Castillo D, Richart C.

Department: Medicina i Cirurgia Bioquímica i Biotecnologia Ciències Mèdiques Bàsiques

e-ISSN: 1422-0067

URV's Author/s: ARAGONÈS BARGALLÓ, GEMMA / Auguet Quintillà, Maria Teresa / BERLANGA BUSTOS, ALBA / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Hernández González, Mercè / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima / SIRVENT CALVERA, JUAN JOSÉ

Keywords: Obesity Nonalcoholic fatty liver disease Mir33a/b* Mir122 nonalcoholic fatty liver disease mir33a/b* mir122

Abstract: Specific miRNA expression profiles have been shown to be associated with nonalcoholic fatty liver disease (NAFLD). We examined the correlation between the circulating levels and hepatic expression of miR122 and miR33a/b*, the key lipid metabolism-related gene expression and the clinicopathological factors of obese women with NAFLD. We measured miR122 and miR33a/b* expression in liver samples from 62 morbidly obese (MO), 30 moderately obese (ModO), and eight normal-weight controls. MiR122 and miR33a/b* expression was analyzed by qRT-PCR. Additionally, miR122 and miR33b* circulating levels were analyzed in 122 women. Hepatic miR33b* expression was increased in MO compared to ModO and controls, whereas miR122 expression was decreased in the MO group compared to ModO. In obese cohorts, miR33b* expression was increased in nonalcoholic steatohepatitis (NASH). Regarding circulating levels, MO patients with NASH showed higher miR122 levels than MO with simple steatosis (SS). These circulating levels are good predictors of histological features associated with disease severity. MO is associated with altered hepatic miRNA expression. In obese women, higher miR33b* liver expression is associated with NASH. Moreover, multiple correlations between miRNAs and the expression of genes related to lipid metabolism were found, that would suggest a miRNA-host gene circuit. Finally, miR122 circulating levels could be included in a panel of different biomarkers to improve accuracy in the non-invasive diagnosis of NASH.

Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14220067

Author's mail: mariasalome.martinez@urv.cat cristobalmanuel.richart@urv.cat danieldel.castillo@urv.cat mariateresa.auguet@urv.cat fatima.sabench@urv.cat merce.hernandez@urv.cat

Author identifier: 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0456-3102 0000-0001-7024-7824 0000-0003-0396-6428 0000-0002-9262-8756

Record's date: 2023-03-11

Journal volume: 17

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1422-0067/17/10/1620

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Molecular Sciences. 17 (10):

APA: Auguet T, Aragonès G, Berlanga A, Guiu-Jurado E, Martí A, Martínez S, Sabench F, Hernández M, Aguilar C, Sirvent JJ, Del Castillo D, Del Castillo D, R (2016). miR33a/miR33b* and miR122 as Possible Contributors to Hepatic Lipid Metabolism in Obese Women with nonalcoholic Fatty Liver Disease. International Journal Of Molecular Sciences, 17(10), -. DOI: 10.3390/ijms17101620

Article's DOI: 10.3390/ijms17101620

Entity: Universitat Rovira i Virgili

Journal publication year: 2016

Publication Type: Journal Publications