Author, as appears in the article.: Blanco J, Tomás-Hernández S, García T, Mulero M, Gómez M, Domingo JL, Sánchez DJ.

Department: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia

URV's Author/s: Blanco Pérez, Jordi / Domingo Roig, José Luis / Gómez Arnaiz, María Mercedes / Mulero Abellán, Miguel / SANCHEZ CERVELLÓ, DOMINGO JOSÉ / TOMAS HERNÁNDEZ, SARA

Keywords: Silver nanoparticles Oxidative stress Insulin pathway Authophagy oxidative stress insulin pathway authophagy

Abstract: The present study was aimed at assessing the impact of AgNPs on the liver of male rats orally exposed to 0, 50, 100 and 200 mg/kg/day of polyvinyl pyrrolidone coated AgNPs (PVP-AgNPs) for 90 days. The induction of apoptotic cell death -by measuring the protein levels of the active form of caspase 3- and the levels of the microtubule-associated protein 1A/1B-light chain (LC3) protein were measured as a marker of the induction of autophagy. PVP-AgNPs caused an increase of the activity of superoxide dismutase (SOD) and catalase (CAT) in the liver of male rats. However, the activity decreased after exposure to high amounts of PVP-AgNPs. Increased protein levels of IRS-1, AKT, GSK3β and mTOR proteins were observed in a dose-dependent manner. However, these proteins showed a decrease at 200 mg/kg/day. The same pattern was observed for the p53, p21 and cleaved caspase 3 protein levels. The current results suggest that the increase of ROS production by PVP-AgNPs stimulated SOD and CAT activity, as well as IRS-1, AKT, mTOR, p53, p21 and caspase 3 as protective mechanisms of cell survival and preserve DNA fidelity. However, cellular damage by excessive ROS production might induce the depletion of these survival mechanisms at 200 mg/kg/day.

Thematic Areas: Toxicology Saúde coletiva Química Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Food science & technology Food science Farmacia Ensino Engenharias iv Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 02786915

Author's mail: jordi.blanco@urv.cat miquel.mulero@urv.cat joseluis.domingo@urv.cat

Author identifier: 0000-0001-8016-0984 0000-0001-6647-9470

Record's date: 2023-02-22

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.sciencedirect.com/science/article/pii/S0278691518301881?via%3Dihub

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Food And Chemical Toxicology. 115 398-404

APA: Blanco J, Tomás-Hernández S, García T, Mulero M, Gómez M, Domingo JL, Sánchez DJ. (2018). Oral exposure to silver nanoparticles increases oxidative stress markers in the liver of male rats and deregulates the insulin signalling pathway and p53 and cleaved caspase 3 protein expression. Food And Chemical Toxicology, 115(), 398-404. DOI: 10.1016/j.fct.2018.03.039

Article's DOI: 10.1016/j.fct.2018.03.039

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

Publication Type: Journal Publications