Author, as appears in the article.: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M.

Department: Bioquímica i Biotecnologia

URV's Author/s: Ardèvol Grau, Anna / Arola Ferrer, Luis Maria / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena

Keywords: Transepithelial activity Gut hormone secretion Food bioactives Ex vivo model Enteroendocrine system gut hormone secretion food bioactives ex vivo model enteroendocrine system

Abstract: The enteroendocrine system coordinates gastrointestinal (GI) tract functionality and the whole organism. However, the scarcity of enteroendocrine cells and their scattered distribution make them difficult to study. Here, we glued segments of the GI wall of pigs to a silicon tube, keeping the apical and the basolateral sides separate. The fact that there was less than 1% of 70-kDa fluorescein isothiocyanate (FITC)-dextran on the basolateral side proved that the gluing was efficient. Since the lactate dehydrogenase leakage at basolateral side was lower than 0.1% (1.40 ± 0.17 nKatals) it proved that the tissue was viable. The intestinal barrier function was maintained as it is in segments mounted in Ussing chambers (the amount of Lucifer Yellow crossing it, was similar between them; respectively, % LY, 0.48 ± 0.13; 0.52 ± 0.09; p > 0.05). Finally, apical treatments with two different extract produced differential basolateral enterohormone secretions (basolateral PYY secretion vs control; animal extract, 0.35 ± 0.16; plant extract, 2.5 ± 0.74; p < 0.05). In conclusion, we report an ex vivo system called "Ap-to-Bas" for assaying vectorial transepithelial processes that makes it possible to work with several samples at the same time. It is an optimal device for enterohormone studies in the intestine.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Planejamento urbano e regional / demografia Odontología Nutrição Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry General agricultural and biological sciences Food science & technology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física Agriculture, multidisciplinary Agriculture Agricultural and biological sciences (miscellaneous) Agricultural and biological sciences (all)

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 00218561

Author's mail: ximena.terra@urv.cat lluis.arola@urv.cat montserrat.pinent@urv.cat anna.ardevol@urv.cat

Author identifier: 0000-0003-1043-5844 0000-0003-2767-1974 0000-0003-3550-5378 0000-0003-0156-7538

Record's date: 2023-02-26

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://pubs.acs.org/doi/10.1021/acs.jafc.8b03046

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Journal Of Agricultural And Food Chemistry. 66 (44): 11622-11629

APA: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M. (2018). Novel ex Vivo Experimental Setup to Assay the Vectorial Transepithelial Enteroendocrine Secretions of Different Intestinal Segments.. Journal Of Agricultural And Food Chemistry, 66(44), 11622-11629. DOI: 10.1021/acs.jafc.8b03046

Article's DOI: 10.1021/acs.jafc.8b03046

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

Publication Type: Journal Publications