Author, as appears in the article.: Leonardo, Sandra; Kiparissis, Sotiris; Rambla-Alegre, Maria; Almarza, Sergi; Roque, Ana; Andree, Karl B.; Christidis, Aris; Flores, Cintia; Caixach, Josep; Campbell, Katrina; Elliott, Christopher T.; Aligizaki, Katerina; Diogene, Jorge; Campas, Monica;

Department: Enginyeria Química

URV's Author/s: CAMPAS HOMS, MONICA

Keywords: Tetrodotoxin Pufferfish Magnetic bead Liquid chromatography-high resolution mass spectrometry (lc-hrms) Lagocephalus sceleratus Juvenile Invasive pufferfish Immunoassay Electrochemical toxin profile toxicity records pufferfish magnetic bead liquid chromatography-high resolution mass spectrometry (lc-hrms) lagocephalus sceleratus juvenile immunoassay identification fish caught electrochemical biosensors analogs

Abstract: Two small Lagocephalus sceleratus juveniles were captured in picarel targeting catches from North Aegean Sea (Greece) in the autumn of 2017. An electrochemical immunosensing tool using magnetic beads as immobilisation support was developed and applied to the rapid screening of tetrodotoxins (TTXs), potent neurotoxins that constitute a food safety hazard when present in seafood. This tool revealed the presence of TTXs in both individuals. Results were compared with those provided by mELISA and LC-HAMS, the latter confirming the presence of TTX. Some of the tissues contained TTX contents close to or above 2 mg/kg. L. sceleratus juveniles had been considered as non-toxic and, to our knowledge, this is the first report of high TTX levels in small L. sceleratus individuals. Such specimens can be mistaken with other edible species, posing a threat to consumers. The availability of low-cost and user-friendly tools for TTXs detection will contribute to guarantee seafood safety.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geociências Food science & technology Food science Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Biotecnología Biodiversidade Astronomia / física Analytical chemistry Administração pública e de empresas, ciências contábeis e turismo

ISSN: 03088146

Author's mail: monica.campas@urv.cat

Author identifier: 0000-0002-1220-7100

Record's date: 2022-07-23

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Food Chemistry. 290 255-262

APA: Leonardo, Sandra; Kiparissis, Sotiris; Rambla-Alegre, Maria; Almarza, Sergi; Roque, Ana; Andree, Karl B.; Christidis, Aris; Flores, Cintia; Caixach, J (2019). Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool. Food Chemistry, 290(), 255-262. DOI: 10.1016/j.foodchem.2019.03.148

Article's DOI: 10.1016/j.foodchem.2019.03.148

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications