Articles producció científica> Medicina i Cirurgia

Methodological constraints in interpreting serum paraoxonase-1 activity measurements: An example from a study in HIV-infected patients

  • Identification data

    Identifier: imarina:5123971
    Handle: http://hdl.handle.net/20.500.11797/imarina5123971
  • Authors:

    Parra, Sandra
    Marsillach, Judit
    Aragones, Gerard
    Rull, Anna
    Beltran-Debon, Raul
    Alonso-Villaverde, Carlos
    Joven, Jorge
    Camps, Jordi
  • Others:

    Author, as appears in the article.: Parra, Sandra; Marsillach, Judit; Aragones, Gerard; Rull, Anna; Beltran-Debon, Raul; Alonso-Villaverde, Carlos; Joven, Jorge; Camps, Jordi;
    Department: Medicina i Cirurgia
    URV's Author/s: Aragonès Bargalló, Gerard / Beltrán Debón, Raúl Alejandro / Camps Andreu, Jorge / Joven Maried, Jorge / RULL AIXA, ANNA
    Keywords: @infoAeu @residentesaeu @uroweb Etiqueta «#» Hashtag
    Abstract: Background. Paraoxonase-1 (PON1) is an antioxidant enzyme that attenuates the production of the monocyte chemoattractant protein-1 (MCP-1) in vitro. Although oxidation and inflammation are closely related processes, the association between PON1 and MCP-1 has not been completely characterised due, probably, to that the current use of synthetic substrates for PON1 measurement limits the interpretation of the data. In the present study, we explored the relationships between the circulating levels of PON1 and MCP-1 in human immunodeficiency virus-infected patients in relation to the multifunctional capabilities of PON1. Methods. We measured selected variables in 227 patients and in a control group of 409 participants. Serum PON1 esterase and lactonase activities were measured as the rates of hydrolysis of paraoxon and of 5-(thiobutyl)-butyrolactone, respectively. Oxidised LDL and MCP-1 concentrations were determined by enzyme-linked immunosorbent assay. High-density lipoproteins cholesterol, apolipoprotein A-I, and C-reactive protein concentrations were measured by standard automated methods. Results. There were significant relationships between PON1 activity and several indices of oxidation and inflammation in control subjects and in infected patients. However, these relationships varied not only with disease status but also on the type of substrate used for PON1 measurement. Conclusion. The present study is a cautionary tale highlighting that results of clinical studies on PON1 may vary depending on the methods used as well as the disease studied. Until more specific methods using physiologically-akin substrates are developed for PON1 measurement, we suggest the simultaneous employment of at least two different substrates in order to improve the reliability of the results obtained. © 2010 Parra et al; licensee BioMed Central Ltd.
    Thematic Areas: Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Ensino Enfermagem Endocrinology, diabetes and metabolism Endocrinology Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry (medical) Biochemistry & molecular biology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1476-511X
    Author's mail: jorge.camps@urv.cat gerard.aragones@urv.cat raul.beltran@urv.cat jorge.joven@urv.cat
    Author identifier: 0000-0002-3165-3640 0000-0001-9691-1906 0000-0003-2749-4541
    Record's date: 2023-02-18
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://lipidworld.biomedcentral.com/articles/10.1186/1476-511X-9-32
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Lipids In Health And Disease. 9 (32): 32-
    APA: Parra, Sandra; Marsillach, Judit; Aragones, Gerard; Rull, Anna; Beltran-Debon, Raul; Alonso-Villaverde, Carlos; Joven, Jorge; Camps, Jordi; (2010). Methodological constraints in interpreting serum paraoxonase-1 activity measurements: An example from a study in HIV-infected patients. Lipids In Health And Disease, 9(32), 32-. DOI: 10.1186/1476-511X-9-32
    Article's DOI: 10.1186/1476-511X-9-32
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2010
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Biochemistry (Medical),Clinical Biochemistry,Endocrinology,Endocrinology, Diabetes and Metabolism,Nutrition & Dietetics
    Saúde coletiva
    Química
    Odontología
    Nutrition & dietetics
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Farmacia
    Ensino
    Enfermagem
    Endocrinology, diabetes and metabolism
    Endocrinology
    Educação física
    Clinical biochemistry
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciência de alimentos
    Biotecnología
    Biodiversidade
    Biochemistry (medical)
    Biochemistry & molecular biology
  • Documents:

  • Cerca a google

    Search to google scholar