Author, as appears in the article.: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus A.

Department: Química Analítica i Química Orgànica

URV's Author/s: Serra Albet, Maria Angels

Keywords: Water-absorption Scaffold Release Polymer testing Polymer degradation Polylactide Polyester Poly(lactide-co-glycolide) (plga) Plga Performance In vitro validation Fibrous scaffolds Erosion Degradation behavior Biocomposites Biocompatibility Bastides

Abstract: Monitoring and understanding the in vitro behaviour of polyester based scaffolds both comprising the study of the hydrolytic degradation and the cell seeding viability is essential to ensure the desired functionality, according to a given biomedical purpose. As a model case to compare the performance of techniques to monitor the in vitro behaviour, poly(lactide-co-glycolide) (PLGA) scaffolds were chosen. The in vitro hydrolytic degradation of PLGA scaffolds was carried out in water and phosphate buffered saline (PBS). The evolution of the mass loss, the molar mass, the thermal properties and the surface morphology were monitored. The hydrolytic degradation media was correspondingly evaluated by means of the study of the pH, the amount of acid released and the conductivity. In addition, the in vitro biocompatibility regarding the cell culture viability was studied under physiological conditions. The cellular adhesion, cellular ability to proliferate on the scaffold, the scaffold inflammatory profile and the effect of the scaffold degradation compounds on the cells were assessed. A comparative analysis of the exploited techniques in terms of promptness of identification, depth of knowledge, simplicity of obtaining results and cost of the technique was implemented. The results showed that, depending on the balance between the interest in ascertaining the trigger of degradation or deep into the knowledge of the causes and effects of cell culture viability, an appropriate plan of analysis of the validation of polyester-based scaffolds could be designed.

Thematic Areas: Química Polymers and plastics Polymer science Organic chemistry Odontología Medicina ii Medicina i Materials science, characterization & testing Materiais Interdisciplinar Engenharias iv Engenharias iii Engenharias ii Engenharias i Ciências biológicas iii Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Astronomia / física

ISSN: 01429418

Author's mail: angels.serra@urv.cat

Author identifier: 0000-0003-1387-0358

Last page: 267

Record's date: 2023-02-18

Journal volume: 66

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0142941817315647

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Polymer Testing. 66 256-267

APA: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus (2018). In vitro validation of biomedical polyester-based scaffolds: Poly(lactide-co-glycolide) as model-case. Polymer Testing, 66(), 256-267. DOI: 10.1016/j.polymertesting.2018.01.027

Article's DOI: 10.1016/j.polymertesting.2018.01.027

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

First page: 256

Publication Type: Journal Publications