Author, as appears in the article.: Siqueira JPZ, Sutton DA, Gené J, García D, Wiederhold N, Guarro J.

Department: Ciències Mèdiques Bàsiques

URV's Author/s: García Sánchez, Dania / Gené Díaz, Josepa / Guarro Artigas, Josep / ZEN SIQUEIRA, JOAO PAULO

Keywords: Taxonomy Phylogenetic analysis Eurotium Aspergillus Antifungal susceptibility

Abstract: The diversity of Aspergillus species in clinical samples is continuously increasing. Species under the former name Eurotium, currently accommodated in section Aspergillus of the genus Aspergillus, are xerophilic fungi widely found in the human environment and able to grow on substrates with low water activity. However, their prevalence in the clinical setting is poorly known. We have studied the presence of these species in a set of clinical samples from the United States using a multilocus sequence analysis based on the internal transcribed spacer (ITS) region of the rRNA, and fragments of the genes β-tubulin (BenA), calmodulin (CaM), and polymerase II second largest subunit (RPB2). A total of 25 isolates were studied and identified as follows: A. montevidensis (44%), A. chevalieri (36%), A. pseudoglaucus (8%), and A. costiformis (4%). A new species Aspergillus microperforatus is also proposed, which represented 8% of the isolates studied and is characterized by uniseriate conidial heads, subglobose to pyriform vesicles, rough conidia, globose to subglobose cleistothecia, and lenticular and smooth ascospores. The in vitro antifungal activity of eight clinically available antifungals was also determined against these isolates, with the echinocandins and posaconazole having the most potent activity.

Thematic Areas: Zootecnia / recursos pesqueiros Veterinary sciences Veterinary (miscellaneous) Saúde coletiva Química Odontología Mycology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases Geografía General veterinary Farmacia Engenharias iii Engenharias ii Engenharias i Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 13693786

Author's mail: josep.guarro@urv.cat josepa.gene@urv.cat dania.garcias@urv.cat

Author identifier: 0000-0002-7839-7568 0000-0001-6195-9299 0000-0003-2397-1646

Record's date: 2023-02-22

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://academic.oup.com/mmy/article/56/5/541/4372452

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Medical Mycology. 56 (5): 541-550

APA: Siqueira JPZ, Sutton DA, Gené J, García D, Wiederhold N, Guarro J. (2018). Species of Aspergillus section Aspergillus from clinical samples in the United States. Medical Mycology, 56(5), 541-550. DOI: 10.1093/mmy/myx085

Article's DOI: 10.1093/mmy/myx085

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

Publication Type: Journal Publications