Author, as appears in the article.: Hubka V, Nováková A, Jurjević Z, Sklenář F, Frisvad JC, Houbraken J, Arendrup MC, Jørgensen KM, Siqueira JPZ, Gené J, Kolařík M

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Gené Díaz, Josepa / ZEN SIQUEIRA, JOAO PAULO

Keywords: Multispecies coalescence model Indoor fungi Cave mycobiota Bioprospecting Aspergillus Antioxidant compounds Antifungal susceptibility testing indoor fungi cave mycobiota bioprospecting antioxidant compounds antifungal susceptibility testing

Abstract: Aspergillus candidus is a species frequently isolated from stored grain, food, indoor environments, soil and occasionally also from clinical material. Recent bioprospecting studies highlighted the potential of using A. candidus and its relatives in various industrial sectors as a result of their significant production of enzymes and bioactive compounds. A high genetic variability was observed among A. candidus isolates originating from various European countries and the USA, that were mostly isolated from indoor environments, caves and clinical material. The A. candidus sensu lato isolates were characterized by DNA sequencing of four genetic loci, and agreement between molecular species delimitation results, morphological characters and exometabolite spectra were studied. Classical phylogenetic methods (maximum likelihood, Bayesian inference) and species delimitation methods based on the multispecies coalescent model supported recognition of up to three species in A. candidus sensu lato. After evaluation of phenotypic data, a broader species concept was adopted, and only one new species, Aspergillus dobrogensis, was proposed. This species is represented by 22 strains originating from seven countries (ex-type strain CCF 4651T=NRRL 62821T=IBT 32697T=CBS 143370T) and its differentiation from A. candidus is relevant for bioprospecting studies because these species have different exometabolite profiles. Evaluation of the antifungal susceptibility of section Candidi members to six antifungals using the reference EUCAST method showed that all species have low minimum inhibitory concentrations for all tested antifungals. These results suggest applicability of a wide spectrum of antifungal agents for treatment of infections caused by species from section Candidi.

Thematic Areas: Saúde coletiva Química Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iii Engenharias ii Ecology, evolution, behavior and systematics Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14665026

Author's mail: josepa.gene@urv.cat

Author identifier: 0000-0001-6195-9299

Record's date: 2023-02-22

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002583

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Systematic And Evolutionary Microbiology. 68 (4): 995-1011

APA: Hubka V, Nováková A, Jurjević Z, Sklenář F, Frisvad JC, Houbraken J, Arendrup MC, Jørgensen KM, Siqueira JPZ, Gené J, Kolařík M (2018). Polyphasic data support the splitting of Aspergillus candidus into two species; proposal of Aspergillus dobrogensis sp. nov.. International Journal Of Systematic And Evolutionary Microbiology, 68(4), 995-1011. DOI: 10.1099/ijsem.0.002583

Article's DOI: 10.1099/ijsem.0.002583

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

Publication Type: Journal Publications