Author, as appears in the article.: Formentín P, Catalán, Pol L, Fernández-Castillejo S, Solà R, Marsal L

Department: Medicina i Cirurgia Enginyeria Electrònica, Elèctrica i Automàtica

URV's Author/s: Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA / Marsal Garví, Luis Francisco / Solà Alberich, Rosa Maria

Keywords: Surface properties Nanoporous anodic alumina Macroporous silicon Fibronectin Endothelial cells Collagen adhesion, morphology and proliferation nanoporous anodic alumina macroporous silicon fibronectin endothelial cells collagen adhesion, morphology and proliferation

Abstract: Background The ability to direct the cellular response by means of biomaterial surface topography is important for biomedical applications. Substrate surface topography has been shown to be an effective cue for the regulation of cellular response. Here, the response of human aortic endothelial cells to nanoporous anodic alumina and macroporous silicon with collagen and fibronectin functionalization has been studied. Methods Confocal microscopy and scanning electron microscopy were employed to analyse the effects of the material and the porosity on the adhesion, morphology, and proliferation of the cells. Cell spreading and filopodia formation on macro- and nanoporous material was characterized by atomic force microscopy. We have also studied the influence of the protein on the adhesion. Results It was obtained the best results when the material is functionalized with fibronectin, regarding cells adhesion, morphology, and proliferation. Conclusion These results permit to obtain chemical modified 3D structures for several biotechnology applications such as tissue engineering, organ-on-chip or regenerative medicine.

Thematic Areas: Molecular biology Medicina i Environmental engineering Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotechnology & applied microbiology Biomedical engineering Biochemical research methods

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 17541611

Author's mail: ursula.catalan@urv.cat lluis.marsal@urv.cat rosa.sola@urv.cat

Author identifier: 0000-0001-8884-9823 0000-0002-5976-1408 0000-0002-8359-235X

Record's date: 2023-02-18

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://jbioleng.biomedcentral.com/articles/10.1186/s13036-018-0111-x

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Journal Of Biological Engineering. 12 (21): 21-

APA: Formentín P, Catalán, Pol L, Fernández-Castillejo S, Solà R, Marsal L (2018). Collagen and fibronectin surface modification of nanoporous anodic alumina and macroporous silicon for endothelial cell cultures. Journal Of Biological Engineering, 12(21), 21-. DOI: 10.1186/s13036-018-0111-x

Article's DOI: 10.1186/s13036-018-0111-x

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

Publication Type: Journal Publications