Author, as appears in the article.: Sunyer-Figueres M., Wang C., Mas A.

Department: Bioquímica i Biotecnologia

URV's Author/s: Mas Baron, Alberto

Keywords: Wine yeasts Heat Dimethyl dicarbonate (dmdc) Cell lysis heat dimethyl dicarbonate (dmdc) cell lysis

Abstract: During wine production, some yeasts enter a Viable But Not Culturable (VBNC) state, which may influence the quality and stability of the final wine through remnant metabolic activity or by resuscitation. Culture-independent techniques are used for obtaining an accurate estimation of the number of live cells, and quantitative PCR could be the most accurate technique. As a marker of cell viability, rRNA was evaluated by analyzing its stability in dead cells. The species-specific stability of rRNA was tested in Saccharomyces cerevisiae, as well as in three species of non-Saccharomyces yeast (Hanseniaspora uvarum, Torulaspora delbrueckii and Starmerella bacillaris). High temperature and antimicrobial dimethyl dicarbonate (DMDC) treatments were efficient in lysing the yeast cells. rRNA gene and rRNA (as cDNA) were analyzed over 48 h after cell lysis by quantitative PCR. The results confirmed the stability of rRNA for 48 h after the cell lysis treatments. To sum up, rRNA may not be a good marker of cell viability in the wine yeasts that were tested.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Safety, risk, reliability and quality Química Odontología Nutrição Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Geociências General medicine Food science & technology Food science Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade

ISSN: 01681605

Author's mail: albert.mas@urv.cat

Author identifier: 0000-0002-0763-1679

Last page: 4

Record's date: 2023-02-18

Journal volume: 270

Papper version: info:eu-repo/semantics/submittedVersion

Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0168160518300369

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Food Microbiology. 270 1-4

APA: Sunyer-Figueres M., Wang C., Mas A. (2018). Analysis of ribosomal RNA stability in dead cells of wine yeast by quantitative PCR. International Journal Of Food Microbiology, 270(), 1-4. DOI: 10.1016/j.ijfoodmicro.2018.01.020

Article's DOI: 10.1016/j.ijfoodmicro.2018.01.020

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

First page: 1

Publication Type: Journal Publications