Author, as appears in the article.: Antonelli F, Casciati A, Tanori M, Tanno B, Linares-Vidal MV, Serra N, Bellés M, Pannicelli A, Saran A, Pazzaglia S.

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Bellés Mateu, Montserrat / Linares Vidal, M. Victoria

Keywords: Tlx nuclear receptor Sonic hedgehog pathway Notch pathway Neurogenesis Hippocampal neurogenesis and neuronal lineage differentiation Heterozygous mice Expression profiles of neurogenesis-related genes Dentate gyrus Behavioral effects tlx nuclear receptor notch pathway hippocampal neurogenesis and neuronal lineage differentiation expression profiles of neurogenesis-related genes behavioral effects

Abstract: Many genes controlling neuronal development also regulate adult neurogenesis. We investigated in vivo the effect of Sonic hedgehog (Shh) signaling activation on patterning and neurogenesis of the hippocampus and behavior of Patched1 (Ptch1) heterozygous mice (Ptch1+/−). We demonstrated for the first time, that Ptch1+/− mice exhibit morphological, cellular and molecular alterations in the dentate gyrus (DG), including elongation and reduced width of the DG as well as deregulations at multiple steps during lineage progression from neural stem cells to neurons. By using stage-specific cellular markers, we detected reduction of quiescent stem cells, newborn neurons and astrocytes and accumulation of proliferating intermediate progenitors, indicative of defects in the dynamic transition among neural stages. Phenotypic alterations in Ptch1+/− mice were accompanied by expression changes in Notch pathway downstream components and TLX nuclear receptor, as well as perturbations in inflammatory and synaptic networks and mouse behavior, pointing to complex biological interactions and highlighting cooperation between Shh and Notch signaling in the regulation of neurogenesis.

Thematic Areas: Neurosciences Molecular biology Medicina ii Ciências biológicas ii Cellular and molecular neuroscience

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 16625099

Author's mail: montserrat.belles@urv.cat mvictoria.linares@urv.cat

Author identifier: 0000-0002-9282-2989 0000-0003-4170-4197

Record's date: 2023-02-22

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.frontiersin.org/articles/10.3389/fnmol.2018.00168/full

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Frontiers In Molecular Neuroscience. 11 (168): 168-

APA: Antonelli F, Casciati A, Tanori M, Tanno B, Linares-Vidal MV, Serra N, Bellés M, Pannicelli A, Saran A, Pazzaglia S. (2018). Alterations in Morphology and Adult Neurogenesis in the Dentate Gyrus of Patched1 Heterozygous Mice.. Frontiers In Molecular Neuroscience, 11(168), 168-. DOI: 10.3389/fnmol.2018.00168

Article's DOI: 10.3389/fnmol.2018.00168

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

Publication Type: Journal Publications