Author, as appears in the article.: Espinel-Ingroff A, Abreu D, Almeida-Paes R, Brilhante R, Chakrabarti A, Chowdhary A, Hagen F, Córdoba S, Gonzalez G, Govender N, Guarro J, Johnson E, Kidd S, Pereira S, Rodrigues A, Rozental S, Szeszs M, Ballesté Alaniz R, Bonifaz A, Bonfietti L, Borba-Santos L, Capilla J, Colombo A, Dolande M, Isla M, Melhem M, Mesa-Arango A, Oliveira M, Panizo M, Pires de Camargo Z, Zancope-Oliveira R, Meis J, Turnidge J

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Capilla Luque, Javier / Guarro Artigas, Josep

Keywords: Sporothrix Molecular methods Ecvs Antifungal resistance sporothrix ecvs antifungal resistance

Abstract: © 2017 American Society for Microbiology. All Rights Reserved. Clinical and Laboratory Standards Institute (CLSI) conditions for testing the susceptibilities of pathogenic Sporothrix species to antifungal agents are based on a collaborative study that evaluated five clinically relevant isolates of Sporothrix schenckii sensu lato and some antifungal agents. With the advent of molecular identification, there are two basic needs: to confirm the suitability of these testing conditions for all agents and Sporothrix species and to establish species-specific epidemiologic cutoff values (ECVs) or breakpoints (BPs) for the species. We collected available CLSI MICs/minimal effective concentrations (MECs) of amphotericin B, five triazoles, terbinafine, flucytosine, and caspofungin for 301 Sporothrix schenckii sensu stricto, 486 S. brasiliensis, 75 S. globosa, and 13 S. mexicana molecularly identified isolates. Data were obtained in 17 independent laboratories (Australia, Europe, India, South Africa, and South and North America) using conidial inoculum suspensions and 48 to 72 h of incubation at 35°C. Sufficient and suitable data (modal MICs within 2-fold concentrations) allowed the proposal of the following ECVs for S. schenckii and S. brasiliensis, respectively: amphotericin B, 4 and 4 μg/ml; itraconazole, 2 and 2 μg/ml; posaconazole, 2 and 2 μg/ml; and voriconazole, 64 and 32 μg/ml. Ketoconazole and terbinafine ECVs for S. brasiliensis were 2 and 0.12 μg/ml, respectively. Insufficient or unsuitable data precluded the calculation of ketoconazole and terbinafine (or any other antifungal agent) ECVs for S. schenckii, as well as ECVs for S. globosa and S. mexicana. These ECVs could aid the clinician in identifying potentially resistant isolates (non-wild type) less likely to respond to therapy.

Thematic Areas: Saúde coletiva Química Pharmacology (medical) Pharmacology & pharmacy Pharmacology Odontología Nutrição Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Infectious diseases Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 00664804

Author's mail: josep.guarro@urv.cat javier.capilla@urv.cat

Author identifier: 0000-0002-7839-7568 0000-0002-0765-6403

Record's date: 2023-02-18

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://aac.asm.org/content/61/10/e01057-17

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Antimicrobial Agents And Chemotherapy. 61 (10):

APA: Espinel-Ingroff A, Abreu D, Almeida-Paes R, Brilhante R, Chakrabarti A, Chowdhary A, Hagen F, Córdoba S, Gonzalez G, Govender N, Guarro J, Johnson E, (2017). Multicenter, international study of MIC/MEC distributions for definition of epidemiological cutoff values (ECVs) for species of Sporothrix identified by molecular methods. Antimicrobial Agents And Chemotherapy, 61(10), -. DOI: 10.1128/AAC.01057-17

Article's DOI: 10.1128/AAC.01057-17

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

Publication Type: Journal Publications