Author, as appears in the article.: Schreiber E, Alfageme O, Garcia T, González N, Sirvent JJ, Torrente M, Gómez M, Domingo JL

Department: Ciències Mèdiques Bàsiques Psicologia Bioquímica i Biotecnologia

URV's Author/s: Domingo Roig, José Luis / Gómez Arnaiz, María Mercedes / Gonzalez Paradell, Nieves / Torrente Torné, Margarita

Keywords: Sperm Reproductive toxicity Maternal exposure Endocrine disruptors Dienestrol reproductive toxicity maternal exposure endocrine disruptors dienestrol

Abstract: This study was aimed at determining whether dienestrol (DIES) affects reproduction in male offspring of rats following oral maternal exposure during gestation and lactation. Pregnant rats were treated from GD 6 to PND 21. Animals received 0 (control-vehicle), 0.75, 1.5, 3.12, 6.25, 12.5, 50, 75 μg/kg bw/d of DIES. A control group -without vehicle-was also included. High DIES concentrations caused abortions at 75 and 50 μg/kg bw/d, while at 12.5 μg/kg bw/d had still miscarriages. Ten male rats per group were kept alive until PND 90 to ensure sexual maturity. Body and organ weights, anogenital distance (AGD) at PNDs 21 and 90, biochemical and sperm parameters like motility, viability, morphology, spermatozoa and resistant spermatid counts, and histopathology for sexual organs and liver were determined. An increase in organ weight (liver and sexual organs) and a decrease in AGD due to vehicle were found. A reduction of sperm motility and viability, and an increase of abnormal sperm morphology were caused by DIES, which provoked a dose-dependent prostatitis. Maternal exposure to DIES induced toxicity on the reproductive system of the male offspring, which could affect the capacity of fertilization.

Thematic Areas: Toxicology Saúde coletiva Química Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Food science & technology Food science Farmacia Ensino Engenharias iv Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 02786915

Author's mail: nieves.gonzalez@urv.cat margarita.torrente@urv.cat joseluis.domingo@urv.cat

Author identifier: 0000-0003-4134-016X 0000-0002-8901-6345 0000-0001-6647-9470

Last page: 201

Record's date: 2023-02-22

Journal volume: 128

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.sciencedirect.com/science/article/pii/S0278691519302121?via%3Dihub

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Food And Chemical Toxicology. 128 193-201

APA: Schreiber E, Alfageme O, Garcia T, González N, Sirvent JJ, Torrente M, Gómez M, Domingo JL (2019). Oral exposure of rats to dienestrol during gestation and lactation: Effects on the reproductive system of male offspring. Food And Chemical Toxicology, 128(), 193-201. DOI: 10.1016/j.fct.2019.04.013

Article's DOI: 10.1016/j.fct.2019.04.013

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

First page: 193

Publication Type: Journal Publications