Author, as appears in the article.: Pascual-Serrano A; Bladé C; Suárez M; Arola-Arnal A

Department: Bioquímica i Biotecnologia

e-ISSN: 1422-0067

URV's Author/s: Arola Arnal, Anna / PASCUAL SERRANO, AÏDA / Suárez Recio, Manuel

Keywords: Resveratrol Ppar-gamma Polyphenols Polyphenol Mice Metabolic syndrome Hypertrophy Hyperplasia High-fat Gene-expression Flavonoids Brown Adipogenesis Adipocyte Activated receptor-gamma 3t3-l1 cells hypertrophy hyperplasia flavonoids adipogenesis adipocyte

Abstract: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. The development of metabolic complications associated with obesity has been correlated with a failure of white adipose tissue (WAT) to expand. Our group has previously reported that a 12-week administration of grape seed proanthocyanidin extract (GSPE) together with an obesogenic diet mitigated the development of cardiometabolic complications in rats. Using the same cohort of animals, we aim to elucidate whether the prevention of cardiometabolic complications by proanthocyanidins is produced by a healthier expansion of visceral WAT and/or an induction of the browning of WAT. For this, adipocyte size and number in retroperitoneal WAT (rWAT) were determined by histological analyses, and the gene expression levels of markers of adipogenesis, browning, and WAT functionality were quantified by RT-qPCR. The long-term administration of GSPE together with an obesogenic diet expanded rWAT via an increase in the adipocyte number and a preventive decrease in the adipocyte size in a dose-dependent manner. At the molecular level, GSPE seems to induce WAT adipogenesis through the upregulation of peroxisome proliferator-activated receptor (Pparγ) in a Sirtuin 1 (Sirt1)-dependent manner. In conclusion, the healthier visceral WAT expansion induced by proanthocyanidins supplementation may explain the improvement in the cardiometabolic risks associated with obesogenic diets.

Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14220067

Author's mail: anna.arola@urv.cat manuel.suarez@urv.cat

Author identifier: 0000-0001-6529-1345 0000-0003-0122-8253

Record's date: 2023-02-22

Journal volume: 19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1422-0067/19/9/2632

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Molecular Sciences. 19 (9):

APA: Pascual-Serrano A; Bladé C; Suárez M; Arola-Arnal A (2018). Grape seed proanthocyanidins improve white adipose tissue expansion during diet-induced obesity development in rats. International Journal Of Molecular Sciences, 19(9), -. DOI: 10.3390/ijms19092632

Article's DOI: 10.3390/ijms19092632

Entity: Universitat Rovira i Virgili

Journal publication year: 2018

Publication Type: Journal Publications