Author, as appears in the article.: Bravo FI; Mas-Capdevila A; Margalef M; Arola-Arnal A; Muguerza B

Department: Bioquímica i Biotecnologia

URV's Author/s: Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / MARGALEF JORNET, MARIA / Muguerza Marquínez, Maria Begoña

Keywords: Spontaneously hypertensive rats Protein hydrolysates Mass spectrometry Hypertension Ace inhibitory peptides protein hydrolysates mass spectrometry hypertension ace inhibitory peptides

Abstract: © 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Scope: Chicken foot proteins have recently been demonstrated by the group to be a great source of hydrolysates with antihypertensive properties. The aim of this study was to isolate and identify angiotensin I-converting enzyme inhibitory (ACEI) peptides from chicken foot hydrolysate Hpp11 and to test their antihypertensive properties. Methods and Results: Peptides are separated into fractions according to their molecular size and hydrophobicity by ultrafiltration and RP-HPLC, respectively. Subsequent peptide identification in the two fractions that present the highest ACEI activities is carried out by HPLC-MS. Ten of the identified peptides are synthesized and five of them show ACEI (IC 50 ) values lower than 100 µm. The antihypertensive effects of these ACEI peptides after oral administration is evaluated in spontaneously hypertensive rats. The peptides AVFQHNCQE and QVGPLIGRYCG exhibit antihypertensive activity when administered at an oral dose of 10 mg kg −1 body weight. The maximal decrease in systolic blood pressure is recorded 6 h after their administration (−25.07 ± 4.21 and −10.94 ± 1.96 mmHg, respectively). Conclusion: These results suggest that AVFQHNCQE and QVGPLIGRYCG could be used as functional ingredients with antihypertensive effects, although it would be necessary to perform bioavailability and clinical studies to demonstrate their efficiency in humans.

Thematic Areas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1613-4125

Author's mail: franciscaisabel.bravo@urv.cat begona.muguerza@urv.cat anna.arola@urv.cat

Author identifier: 0000-0002-6468-3088 0000-0001-7384-8588 0000-0001-6529-1345

Record's date: 2023-02-18

Journal volume: 63

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201801176

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Molecular Nutrition & Food Research. 63 (12): e1801176-

APA: Bravo FI; Mas-Capdevila A; Margalef M; Arola-Arnal A; Muguerza B (2019). Novel Antihypertensive Peptides Derived from Chicken Foot Proteins. Molecular Nutrition & Food Research, 63(12), e1801176-. DOI: 10.1002/mnfr.201801176

Article's DOI: 10.1002/mnfr.201801176

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications