Author, as appears in the article.: Alcaide-Hidalgo JM; Margalef M; Bravo FI; Muguerza B; López-Huertas E

Department: Bioquímica i Biotecnologia

URV's Author/s: Bravo Vázquez, Francisca Isabel / MARGALEF JORNET, MARIA / Muguerza Marquínez, Maria Begoña

Keywords: Spontaneously hypertensive rats Peptides Olive oil Hypertension Ace peptides olive oil hypertension ace

Abstract: © 2019 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism Background & aims: The peptide and protein composition of olive oil is mostly unknown and the few studies available have not focused on the study of its low molecular weight peptides. We hypothesised that olive oil could naturally contain low molecular weight peptides with antihypertensive effect. Methods: We produced virgin olive oil (unfiltered, var. Picual) and obtained a water-soluble peptide extract. We fractionated the peptide extract by FPLC and studied its angiotensin converting enzyme (ACE) inhibitory activity. We studied the antihypertensive effect of olive oil peptides on the systolic blood pressure (SBP) and diastolic blood pressure (DBP) using an animal model of hypertension (spontaneously hypertensive rats, SHR). The animals were randomly distributed into 3 study groups (n = 8 per group) and received an oral dose of olive oil peptides (0.425 mg/kg of BW), or a dose of Captopril (50 mg/kg of BW) or water. SBP and DBP were registered in the rats before administration and a at 2, 4, 6, 8, 24 and 48 h post-administration of the corresponding dose. Results: The peptide extract and FPLC purified fractions possessed angiotensin converting enzyme (ACE) inhibitory activity. Acute oral administration of olive oil water-soluble extract produced an average blood pressure reduction of 10 mmHg at 4 h (P < 0.01) and reached a maximum antihypertensive effect of 20 mmHg at 6 h, compared with baseline. Conclusion: Unfiltered virgin olive oil contains peptides and a water-soluble extract obtained from this oil possesses ACE inhibitory activity and in vivo antihypertensive effect.

Thematic Areas: Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Educação física Critical care and intensive care medicine Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología

ISSN: 02615614

Author's mail: franciscaisabel.bravo@urv.cat begona.muguerza@urv.cat

Author identifier: 0000-0002-6468-3088 0000-0001-7384-8588

Record's date: 2023-02-18

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0261561419302274

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Clinical Nutrition. 39 (4): 1242-1249

APA: Alcaide-Hidalgo JM; Margalef M; Bravo FI; Muguerza B; López-Huertas E (2020). Virgin olive oil (unfiltered) extract contains peptides and possesses ACE inhibitory and antihypertensive activity. Clinical Nutrition, 39(4), 1242-1249. DOI: 10.1016/j.clnu.2019.05.016

Article's DOI: 10.1016/j.clnu.2019.05.016

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications