Articles producció científica> Bioquímica i Biotecnologia

Grape-seed proanthocyanidins are able to reverse intestinal dysfunction and metabolic endotoxemia induced by a cafeteria diet in wistar rats

  • Identification data

    Identifier: imarina:5658592
    Handle: http://hdl.handle.net/20.500.11797/imarina5658592
  • Authors:

    González-Quilen C
    Gil-Cardoso K
    Ginés I
    Beltrán-Debón R
    Pinent M
    Ardévol A
    Terra X
    Blay M
  • Others:

    Author, as appears in the article.: González-Quilen C; Gil-Cardoso K; Ginés I; Beltrán-Debón R; Pinent M; Ardévol A; Terra X; Blay M
    Department: Bioquímica i Biotecnologia
    e-ISSN: 2072-6643
    URV's Author/s: Ardèvol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / GONZALEZ QUILEN, CARLOS ALBERTO / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
    Keywords: Ussing chamber Transepithelial electrical resistance Tight junction Supplementation Rich Obesity Liver Insulin-resistance Inflammation High-fat diet Gut microbiota Gut Flavan-3-ol Extract Barrier function ussing chamber tight junction obesity inflammation gut flavan-3-ol
    Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. We evaluated the effectiveness of pharmacological doses of grape-seed proanthocyanidin extract (GSPE) in reversing intestinal barrier alterations and local inflammation in female Wistar rats fed a long-term obesogenic diet. Animals were fed a 17-week cafeteria diet (CAF diet), supplemented with daily GSPE doses (100 or 500 mg kg-1 body weight) during the final two weeks. CAF diet enhanced the intestinal permeation of an orally administered marker (ovalbumin, OVA) and increased the plasma levels of tumor necrosis factor-α (TNF-α) and lipopolysaccharides (LPS) in 2-3-fold. Ex vivo Ussing chamber assays showed a 55-70% reduction in transepithelial electrical resistance (TEER) and increased the TNF-α secretions in both small and large intestinal sections with a 25-fold increment in the ileum. Ileal tissues also presented a 4-fold increase of myeloperoxidase (MPO) activity. Both GSPE-treatments were able to restitute TEER values in the ileum and colon and to reduce plasma LPS to basal levels without a dose-dependent effect. However, effects on the OVA permeation and TNF-α secretion were dose and section-specific. GSPE also reduced ileal MPO activity and upregulated claudin 1 gene expression. This study provides evidence of the efficacy of GSPE-supplementation ameliorating diet-induced intestinal dysfunction and metabolic endotoxemia when administered at the end of a long-term obesogenic diet.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 20726643
    Author's mail: ximena.terra@urv.cat raul.beltran@urv.cat montserrat.pinent@urv.cat mteresa.blay@urv.cat anna.ardevol@urv.cat
    Author identifier: 0000-0003-1043-5844 0000-0001-9691-1906 0000-0003-3550-5378 0000-0002-6256-9847 0000-0003-0156-7538
    Record's date: 2023-02-26
    Journal volume: 11
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2072-6643/11/5/979
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Nutrients. 11 (5):
    APA: González-Quilen C; Gil-Cardoso K; Ginés I; Beltrán-Debón R; Pinent M; Ardévol A; Terra X; Blay M (2019). Grape-seed proanthocyanidins are able to reverse intestinal dysfunction and metabolic endotoxemia induced by a cafeteria diet in wistar rats. Nutrients, 11(5), -. DOI: 10.3390/nu11050979
    Article's DOI: 10.3390/nu11050979
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2019
    Publication Type: Journal Publications
  • Keywords:

    Food Science,Nutrition & Dietetics,Nutrition and Dietetics
    Ussing chamber
    Transepithelial electrical resistance
    Tight junction
    Supplementation
    Rich
    Obesity
    Liver
    Insulin-resistance
    Inflammation
    High-fat diet
    Gut microbiota
    Gut
    Flavan-3-ol
    Extract
    Barrier function
    ussing chamber
    tight junction
    obesity
    inflammation
    gut
    flavan-3-ol
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Psicología
    Planejamento urbano e regional / demografia
    Nutrition and dietetics
    Nutrition & dietetics
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science
    Farmacia
    Engenharias iv
    Engenharias ii
    Enfermagem
    Educação física
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
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