Author, as appears in the article.: Ramos-Lopez O; Riezu-Boj JI; Milagro FI; Zulet MA; Santos JL; Martinez JA and MENA project

Department: Bioquímica i Biotecnologia

URV's Author/s: Babio Sánchez, Nancy Elvira / Salas Salvadó, Jorge

Keywords: Smell Or4d2 Or2y1 Olfactory system Epigenetics Diet or4d2 or2y1 olfactory system epigenetics diet

Abstract: © 2019 The Author(s). Background: Olfaction is an important sense influencing food preferences, appetite, and eating behaviors. This hypothesis-driven study aimed to assess associations between olfactory pathway gene methylation signatures, obesity features, and dietary intakes. Methods: A nutriepigenomic analysis was conducted in 474 adults from the Methyl Epigenome Network Association (MENA) project. Anthropometric measurements, clinical data, and serum metabolic profiles of the study population were obtained from structured databases of the MENA cohorts. Habitual dietary intake was assessed using a validated semiquantitative food frequency questionnaire. DNA methylation was measured in circulating white blood cells by microarray (Infinium Human Methylation 450 K BeadChips). FDR values (p < 0.0001) were used to select those CpGs that showed the best correlation with body mass index (BMI) and waist circumference (WC). Pathway analyses involving the characterization of genes involved in the olfactory transduction system were performed using KEGG and pathDIP reference databases. Results: Overall, 15 CpG sites at olfactory pathway genes were associated with BMI (p < 0.0001) and WC (p < 0.0001) after adjustments for potential confounding factors. Together, methylation levels at the15 CpG sites accounted for 22% and 20% of the variability in BMI and WC (r 2 = 0.219, p < 0.001, and r 2 = 0.204, p < 0.001, respectively). These genes encompassed olfactory receptors (OR4D2, OR51A7, OR2T34, and OR2Y1) and several downstream signaling molecules (SLC8A1, ANO2, PDE2A, CALML3, GNG7, CALML6, PRKG1, and CAMK2D), which significantly regulated odor detection and signal transduction processes within the complete olfactory cascade, as revealed by pathway enrichment analyses (p = 1.94 × 10-10). Moreover, OR4D2 and OR2Y1 gene methylation patterns strongly correlated with daily intakes of total energy (p < 0.0001), carbohydrates (p < 0.0001), protein (p < 0.0001), and fat (p < 0.0001). Conclusions: The results of this study suggest novel relationships between olfactory pathway gene methylation signatures, obesity indices, and dietary intakes.

Thematic Areas: Zootecnia / recursos pesqueiros Nutrition & dietetics Nutrição Medicina ii Medicina i Interdisciplinar Genetics & heredity Genetics Endocrinology, diabetes and metabolism Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 15558932

Author's mail: nancy.babio@urv.cat jordi.salas@urv.cat

Author identifier: 0000-0003-3527-5277 0000-0003-2700-7459

Record's date: 2023-02-18

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://genesandnutrition.biomedcentral.com/articles/10.1186/s12263-019-0635-9

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Genes And Nutrition. 14 (1): 11-

APA: Ramos-Lopez O; Riezu-Boj JI; Milagro FI; Zulet MA; Santos JL; Martinez JA and MENA project (2019). Associations between olfactory pathway gene methylation marks, obesity features and dietary intakes. Genes And Nutrition, 14(1), 11-. DOI: 10.1186/s12263-019-0635-9

Article's DOI: 10.1186/s12263-019-0635-9

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications