Author, as appears in the article.: Ginés I, Gil-Cardoso K, Serrano J, Casanova-Marti À, Lobato M, Terra X, Blay MT, Ardévol A, Pinent M

Department: Bioquímica i Biotecnologia

e-ISSN: 2073-4425

URV's Author/s: Ardèvol Grau, Anna / Blay Olivé, Maria Teresa / CASANOVA MARTÍ, ANGELA / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena

Keywords: Rat Proanthocyanidin Lpl Long-lasting effect Adiposity proanthocyanidin lpl long-lasting effect keywords adiposity

Abstract: A dose of proanthocyanidins with satiating properties proved to be able to limit body weight increase several weeks after administration under exposure to a cafeteria diet. Here we describe some of the molecular targets and the duration of the effects. We treated rats with 500 mg grape seed proanthocyanidin extract (GSPE)/kg BW for ten days. Seven or seventeen weeks after the last GSPE dose, while animals were on a cafeteria diet, we used reverse transcriptase-polymerase chain reaction (RT-PCR) to measure the mRNA of the key energy metabolism enzymes from the liver, adipose depots and muscle. We found that a reduction in the expression of adipose Lpl might explain the lower amount of adipose tissue in rats seven weeks after the last GSPE dose. The liver showed increased expression of Cpt1a and Hmgs2 together with a reduction in Fasn and Dgat2. In addition, muscle showed a higher fatty oxidation (Oxct1 and Cpt1b mRNA). However, after seventeen weeks, there was a completely different gene expression pattern. At the conclusion of the study, seven weeks after the last GSPE administration there was a limitation in adipose accrual that might be mediated by an inhibition of the gene expression of the adipose tissue Lpl. Concomitantly there was an increase in fatty acid oxidation in liver and muscle.

Thematic Areas: Medicina ii Medicina i Matemática / probabilidade e estatística Genetics (clinical) Genetics & heredity Genetics Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência da computação Biotecnología Biodiversidade

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 20734425

Author's mail: ximena.terra@urv.cat montserrat.pinent@urv.cat mteresa.blay@urv.cat anna.ardevol@urv.cat

Author identifier: 0000-0003-1043-5844 0000-0003-3550-5378 0000-0002-6256-9847 0000-0003-0156-7538

Record's date: 2023-02-26

Journal volume: 10

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2073-4425/10/8/598

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Genes. 10 (8):

APA: Ginés I, Gil-Cardoso K, Serrano J, Casanova-Marti À, Lobato M, Terra X, Blay MT, Ardévol A, Pinent M (2019). Proanthocyanidins Limit Adipose Accrual Induced by a Cafeteria Diet, Several Weeks after the End of the Treatment.. Genes, 10(8), -. DOI: 10.3390/genes10080598

Article's DOI: 10.3390/genes10080598

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications