Author, as appears in the article.: Nguyen DHK, Loebbe C, Linklater DP, Xu X, Vrancken N, Katkus T, Juodkazis S, Maclaughlin S, Baulin V, Crawford RJ, Ivanova EP
Department: Enginyeria Química
URV's Author/s: Baulin, Vladimir
Keywords: Surfaces Staphylococcus-aureus Roughness Pseudomonas-aeruginosa Infectious-diseases society
Abstract: Nanostructured mechano-bactericidal surfaces represent a promising technology to prevent the incidence of microbial contamination on a variety of surfaces and to avoid bacterial infection, particularly with antibiotic resistant strains. In this work, a regular array of silicon nanopillars of 380 nm height and 35 nm diameter was used to study the release of bacterial cell debris off the surface, following inactivation of the cell due to nanostructure-induced rupture. It was confirmed that substantial bactericidal activity was achieved against Gram-negative Pseudomonas aeruginosa (85% non-viable cells) and only modest antibacterial activity towards Staphylococcus aureus (8% non-viable cells), as estimated by measuring the proportions of viable and non-viable cells via fluorescence imaging. In situ time-lapse AFM scans of the bacteria-nanopillar interface confirmed the removal rate of the dead P. aeruginosa cells from the surface to be approximately 19 minutes per cell, and approximately 11 minutes per cell for dead S. aureus cells. These results highlight that the killing and dead cell detachment cycle for bacteria on these substrata are dependant on the bacterial species and the surface architecture studied and will vary when these two parameters are altered. The outcomes of this work will enhance the current understanding of antibacterial nanostructures, and impact upon the development and implementation of next-generation implants and medical devices.
Thematic Areas: Química Physics, applied Odontología Nanoscience and nanotechnology Nanoscience & nanotechnology Medicina ii Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) Materiais Matemática / probabilidade e estatística Interdisciplinar General materials science Farmacia Engenharias iv Engenharias iii Engenharias ii Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Chemistry, multidisciplinary Biotecnología Biodiversidade Astronomia / física
ISSN: 20403364
Author's mail: vladimir.baulin@urv.cat
Author identifier: 0000-0003-2086-4271
Record's date: 2023-02-18
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Nanoscale. 11 (35): 16455-16462
APA: Nguyen DHK, Loebbe C, Linklater DP, Xu X, Vrancken N, Katkus T, Juodkazis S, Maclaughlin S, Baulin V, Crawford RJ, Ivanova EP (2019). The idiosyncratic self-cleaning cycle of bacteria on regularly arrayed mechano-bactericidal nanostructures. Nanoscale, 11(35), 16455-16462. DOI: 10.1039/c9nr05923g
Article's DOI: 10.1039/c9nr05923g
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications