Author, as appears in the article.: Sok V, Fragoso A

Department: Enginyeria Química

URV's Author/s: Fragoso Sierra, Alex

Keywords: Water Soil Sensors Rice Pollutant Phenol Organophosphorus Nanostructures Nanomaterial Irrigation Immobilization Horseradish-peroxidase Herbicide Enzyme Elisa Electrochemical detection Contamination Carbamate Alkaline-phosphatase

Abstract: Glyphosate [N-(phosphonomethyl)glycine] is the most frequently used herbicide to date. Due to its indiscriminate use, it has become a globally occurring pollutant of surface waters. A biosensor for glyphosate is described here that consists of a carbon nano-onion/tyrosinase conjugate immobilized in a chitosan matrix on a screen-printed electrode. The analytical principle is based on the inhibition of the enzyme tyrosinase by glyphosate. L-DOPA is used as the enzyme substrate. The presence of the carbon nano-onions has a beneficial effect on the sensitivity of the assay. Glyphosate can be amperometrically quantified in the 0.015 to 10 μM concentration range and with a 6.5 nM (1.1 μg L-1) detection limit. The biosensor is stable more than 2 months at 4 °C. It was applied to the detection of glyphosate in water and soil samples taken from irrigation of a rice field after aerial application. Results were in good agreement with data obtained by a commercial ELISA. Graphical abstract A highly sensitive amperometric biosensor for glyphosate is reported, based on the covalent immobilization of a carbon nano-onion/tyrosinase conjugate on a chitosan matrix.

Thematic Areas: Zootecnia / recursos pesqueiros Química Materiais Interdisciplinar Geociências Engenharias iv Engenharias ii Ciências biológicas i Ciências ambientais Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry

ISSN: 00263672

Author's mail: alex.fragoso@urv.cat

Author identifier: 0000-0003-4839-6094

Record's date: 2023-02-22

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Microchimica Acta. 186 (8): 569-569

APA: Sok V, Fragoso A (2019). Amperometric biosensor for glyphosate based on the inhibition of tyrosinase conjugated to carbon nano-onions in a chitosan matrix on a screen-printed electrode. Microchimica Acta, 186(8), 569-569. DOI: 10.1007/s00604-019-3672-6

Article's DOI: 10.1007/s00604-019-3672-6

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications