Author, as appears in the article.: Aragonès G, Colom-Pellicer M, Aguilar C, Guiu-Jurado E, Martínez S, Sabench F, Antonio Porras J, Riesco D, Del Castillo D, Richart C, Auguet T

Department: Medicina i Cirurgia

URV's Author/s: Aguilar Crespillo, Carmen Isabel / ARAGONÈS BARGALLÓ, GEMMA / Auguet Quintillà, Maria Teresa / Colom Pellicer, Marina / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima

Keywords: Hashtag Etiqueta «#» @uroweb @residentesaeu @infoAeu

Abstract: Non-alcoholic fatty liver disease (NAFLD) causes a wide spectrum of liver damage, from simple steatosis (SS) to cirrhosis. SS and non-alcoholic steatohepatitis (NASH) cannot be distinguished by clinical or laboratory features. Dysregulation of the gut microbiota is involved in NASH pathogenesis. The aim of this study was to assess the relationship between microbiota-derived metabolites and the degrees of NAFLD; also, to investigate whether these metabolites could be included in a panel of NASH biomarkers.We used liquid chromatography coupled to triple-quadrupole-mass spectrometry (LC-QqQ) analysis to quantify choline and its derivatives, betaine, endogenous ethanol, bile acids, short-chain fatty acids and soluble TLR4 in serum from women with normal weight (n?=?29) and women with morbid obesity (MO) (n?=?82) with or without NAFLD. We used real-time polymerase chain reaction (RT-PCR) analysis to evaluate the hepatic and intestinal expression level of all genes studied (TLR2, TLR4, TLR9, LXR?, SREBP1C, ACC1, FAS, PPAR?, CPT1?, CROT, SREBP2, ABCA1, ABCG1 and FXR in the liver; TLR2, TLR4, TLR5, TLR9, GLP-1R, DPP-4, FXR and PPAR? in the jejunum) in 82 women with MO with normal liver histology (NL, n?=?29), SS (n?=?32), and NASH (n?=?21).Hepatic FAS, TLR2, and TLR4 expression were overexpressed in NAFLD patients. TLR2 was overexpressed in NASH patients. In women with MO with NAFLD, we found upregulation of intestinal TLR9 expression and downregulation of intestinal FXR expression in women with NASH. Circulating TMAO, glycocholic acid and deoxycholic acid levels were significantly increased in NAFLD patients. Endogenous circulating ethanol levels were increased in NASH patients in comparison to those in SS patients.These findings suggest that the intestine participates in the progression of NAFLD. Moreover, levels of certain circulating microbiota-related metabolites are associated with NAFLD severity and could be used as a "liquid biopsy" in the noninvasive diagnosis of NASH.

Thematic Areas: Serviço social Saúde coletiva Psicología Nutrition and dietetics Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 03070565

Author's mail: joseantonio.porras@urv.cat mariasalome.martinez@urv.cat marina.colom@urv.cat cristobalmanuel.richart@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat fatima.sabench@urv.cat marina.colom@urv.cat marina.colom@urv.cat

Author identifier: 0000-0001-6418-1822 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X 0000-0002-9262-8756

Paper data publication: 2019/08/06

Last page: 885

Record's date: 2023-09-16

Journal volume: 44

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.nature.com/articles/s41366-019-0430-0#citeas

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Obesity. 44 (4): 875-885

APA: Aragonès G, Colom-Pellicer M, Aguilar C, Guiu-Jurado E, Martínez S, Sabench F, Antonio Porras J, Riesco D, Del Castillo D, Richart C, Auguet T (2020). Circulating microbiota-derived metabolites: a “liquid biopsy?. International Journal Of Obesity, 44(4), 875-885. DOI: 10.1038/s41366-019-0430-0

Article's DOI: doi: 10.1038/s41366-019-0430-0

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

First page: 875

Publication Type: Journal Publications