Author, as appears in the article.: Angeles Morcillo-Parra, Maria; Jose Valera, Maria; Beltran, Gemma; Mas, Albert; Torija, Maria-Jesus;

Department: Bioquímica i Biotecnologia

URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / MORCILLO PARRA, MARÍA DE LOS ÁNGELES / Torija Martínez, María Jesús / VALERA MARTÍNEZ, MARÍA JOSÉ

Keywords: Wine yeast Receptors Pyruvate kinase Oxidative stress Mitochondria Metabolites Melatonin Isomers Glycolysis Gland hormone melatonin Gadph Fermented beverages Family Enzymes Enolase Alcoholic fermentation melatonin glycolysis gadph fermented beverages enolase

Abstract: Melatonin is a bioactive compound that is present in fermented beverages and synthesized by yeast during alcoholic fermentation. Many studies have shown that melatonin interacts with some mammalian proteins, such as sirtuins or orphan receptor family proteins. The aim of this study was to determine the intracellular synthesis profile of melatonin in Saccharomyces cerevisiae and to identify the proteins that may interact with this molecule in yeast cells. Melatonin from fermentation samples was analyzed by liquid chromatography mass spectrometry, and proteins bound to melatonin were immunopurified by melatonin-IgG-Dynabeads. Melatonin was produced intracellularly in the lag phase of yeast growth and was exported to the extracellular media during the stationary phase. During this period, melatonin was bound to six proteins with molecular weights from 55 to 35 kDa. Sequence analysis showed that most proteins shared high levels of homology with glycolytic enzymes. An RNA-binding protein was also identified, the elongation alpha factor, which is related to mitochondria. This study reports for the first time the interaction of melatonin and proteins inside yeast cells. These results highlight the possible role of melatonin as a signal molecule and provide a new perspective for understanding its role in yeast.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geografía Geociências Farmacia Ensino Engenharias iii Engenharias ii Engenharias i Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1664302X

Author's mail: albert.mas@urv.cat mjesus.torija@urv.cat gemma.beltran@urv.cat

Author identifier: 0000-0002-0763-1679 0000-0001-6419-0745 0000-0002-7071-205X

Record's date: 2023-02-18

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.frontiersin.org/articles/10.3389/fmicb.2019.02424/full

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Frontiers In Microbiology. 10 (2424): 2424-

APA: Angeles Morcillo-Parra, Maria; Jose Valera, Maria; Beltran, Gemma; Mas, Albert; Torija, Maria-Jesus; (2019). Glycolytic Proteins Interact With Intracellular Melatonin in Saccharomyces cerevisiae. Frontiers In Microbiology, 10(2424), 2424-. DOI: 10.3389/fmicb.2019.02424

Article's DOI: 10.3389/fmicb.2019.02424

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications