Author, as appears in the article.: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F.;

Department: Enginyeria Electrònica, Elèctrica i Automàtica

e-ISSN: 1424-8220

URV's Author/s: Acosta Capilla, Laura Karen / Ferré Borrull, José / Marsal Garví, Luis Francisco

Keywords: Thrombin Streptavidin Selection Rifs Perspective Nanoporous anodic alumina Molecules Electrochemical detection Biosensing Bind Aptamers streptavidin rifs nanoporous anodic alumina biosensing aptamers

Abstract: Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient manner. These chemical modifications must be well defined. In this work we propose nanoporous anodic alumina (NAA) chemically modified with streptavidin as a platform for aptamer immobilization. The immobilization of biotinylated thrombin binding aptamer (TBA) was monitored in real time by means of reflective interferometric spectroscopy (RIfS). The study has permitted to characterize in real time the path to immobilize TBA on the inner pore walls of NAA. Furthermore, this study provides an accurate label-free method to detect thrombin in real-time with high affinity and specificity.

Thematic Areas: Zootecnia / recursos pesqueiros Química Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Linguística e literatura Letras / linguística Interdisciplinar Instruments & instrumentation Instrumentation Information systems Geografía Geociências Farmacia Engineering, electrical & electronic Engenharias iv Engenharias iii Engenharias ii Engenharias i Electrochemistry Electrical and electronic engineering Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Biochemistry Atomic and molecular physics, and optics Astronomia / física Arquitetura, urbanismo e design Analytical chemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14248220

Author's mail: laurakaren.acosta@urv.cat lluis.marsal@urv.cat josep.ferre@urv.cat laurakaren.acosta@urv.cat

Author identifier: 0000-0002-5976-1408 0000-0002-5210-5133

Record's date: 2023-05-13

Journal volume: 19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1424-8220/19/20/4543

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Sensors. 19 (20):

APA: Pol, Laura; Karen Acosta, Laura; Ferre-Borrull, Josep; Marsal, Lluis F.; (2019). Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection. Sensors, 19(20), -. DOI: 10.3390/s19204543

Article's DOI: 10.3390/s19204543

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications