Author, as appears in the article.: Hereu, Merce; Ramos-Romero, Sara; Marin-Valls, Roser; Amezqueta, Susana; Miralles-Perez, Bernat; Romeu, Marta; Mendez, Lucia; Medina, Isabel; Lluis Torres, Josep;

Department: Ciències Mèdiques Bàsiques

e-ISSN: 2072-6643

URV's Author/s: Miralles Pérez, Bernat / Romeu Ferran, Marta

Keywords: Wistar Weight Prevotella Polyunsaturated fatty-acids Omega-3 pufas Model Microbiota Metabolites Lactobacillus Insulin-resistance Impact Iminosugar Iminocyclitol Gut microbiota Diet D-fagomine Bacteroides Acetate ?-3 pufas weight prevotella iminosugar iminocyclitol gut microbiota d-fagomine bacteroides

Abstract: Some functional food components may help maintain homeostasis by promoting balanced gut microbiota. Here, we explore the possible complementary effects of D-fagomine and omega-3 polyunsaturated fatty acids (omega-3 PUFAs) eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA 1:1) on putatively beneficial gut bacterial strains. Male Sprague-Dawley rats were supplemented with D-fagomine, omega-3 PUFAs, or both, for 23 weeks. Bacterial subgroups were evaluated in fecal DNA by quantitative real-time polymerase chain reaction (qRT-PCR) and short-chain fatty acids were determined by gas chromatography. We found that the populations of the genus Prevotella remained stable over time in animals supplemented with D-fagomine, independently of omega-3 PUFA supplementation. Animals in these groups gained less weight than controls and rats given only omega-3 PUFAs. D-Fagomine supplementation together with omega-3 PUFAs maintained the relative populations of Bacteroides. omega-3 PUFAs alone or combined with D-fagomine reduced the amount of acetic acid and total short-chain fatty acids in feces. The plasma levels of pro-inflammatory arachidonic acid derived metabolites, triglycerides and cholesterol were lower in both groups supplemented with omega-3 PUFAs. The D-fagomine and omega-3 PUFAs combination provided the functional benefits of each supplement. Notably, it helped stabilize populations of Prevotella in the rat intestinal tract while reducing weight gain and providing the anti-inflammatory and cardiovascular benefits of omega-3 PUFAs.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 20726643

Author's mail: bernat.miralles@urv.cat marta.romeu@urv.cat bernat.miralles@urv.cat

Author identifier: 0000-0003-1294-7069 0000-0002-2131-1858 0000-0003-1294-7069

Record's date: 2023-02-18

Journal volume: 11

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2072-6643/11/11/2606

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Nutrients. 11 (11):

APA: Hereu, Merce; Ramos-Romero, Sara; Marin-Valls, Roser; Amezqueta, Susana; Miralles-Perez, Bernat; Romeu, Marta; Mendez, Lucia; Medina, Isabel; Lluis To (2019). Combined Buckwheat D-Fagomine and Fish Omega-3 PUFAs Stabilize the Populations of Gut Prevotella and Bacteroides While Reducing Weight Gain in Rats. Nutrients, 11(11), -. DOI: 10.3390/nu11112606

Article's DOI: 10.3390/nu11112606

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications