Author, as appears in the article.: Ardid-ruiz A; Ibars M; Mena P; Del Rio D; Muguerza B; Arola L; Aragonès G; Suárez M

Department: Bioquímica i Biotecnologia

URV's Author/s: Aragonès Bargalló, Gerard / ARDID RUIZ, ANDREA / Arola Ferrer, Luis Maria / IBARS SERRA, MARIA / Muguerza Marquínez, Maria Begoña / Suárez Recio, Manuel

Keywords: Sirtuin 1 Resveratrol metabolites Receptor Polyphenols Obesity Non-alcoholic fatty liver disease (nafld) Lipids Lipid metabolism Leptin resistance Fatty liver-disease Diet Bioavailability resveratrol metabolites obesity non-alcoholic fatty liver disease (nafld) lipid metabolism leptin resistance

Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. The interaction of leptin with its hepatic longest receptor (OBRb) promotes the phosphorylation of signal transducer and activator of transcription-3 (STAT3), protecting the liver from lipid accumulation. However, leptin signalling is disrupted in hepatic steatosis, causing leptin resistance. One promising strategy to combat this problem is the use of bioactive compounds such as polyphenols. Since resveratrol (RSV) is a modulator of lipid homeostasis in the liver, we investigated whether treatment with different doses of RSV restores appropriate leptin action and fat accumulation in palmitate-induced steatotic human hepatoma (HepG2) cells. Both RSV metabolism and the expression of molecules implicated in leptin signalling were analysed. RSV at a 10 μM concentration was entirely metabolized to resveratrol-3-sulfate after 24 and counteracted leptin resistance by increasing the protein levels of OBRb. In addition, RSV downregulated the expression of lipogenic genes including fatty acid synthase (Fas) and stearoyl-CoA desaturase-1 (Scd1) without any significant change in Sirtuin-1 (SIRT1) enzymatic activity. These results demonstrate that RSV restored leptin sensitivity in a cellular model of hepatic steatosis in a SIRT1-independent manner.

Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14220067

Author's mail: gerard.aragones@urv.cat begona.muguerza@urv.cat lluis.arola@urv.cat manuel.suarez@urv.cat

Author identifier: 0000-0001-7384-8588 0000-0003-2767-1974 0000-0003-0122-8253

Record's date: 2023-02-22

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1422-0067/20/24/6282

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Molecular Sciences. 20 (24):

APA: Ardid-ruiz A; Ibars M; Mena P; Del Rio D; Muguerza B; Arola L; Aragonès G; Suárez M (2019). Resveratrol treatment enhances the cellular response to leptin by increasing OBRB content in palmitate-induced steatotic hepg2 cells. International Journal Of Molecular Sciences, 20(24), -. DOI: 10.3390/ijms20246282

Article's DOI: 10.3390/ijms20246282

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications