Author, as appears in the article.: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M.; Terra, Ximena; Ardeyol, Anna; Pinent, Montserrat; Beltran-Debon, Raul;

Department: Bioquímica i Biotecnologia

URV's Author/s: Ardèvol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena

Keywords: Supplementation Sp1 Receptor Proanthocyanidins Polyphenols Methylation Intestine Gut microbiota Grape seed flavanols Glp-1r Flavanols Epigenetics Dna methylation Diet-induced obesity Cells sp1 methylation intestine glp-1r flavanols epigenetics

Abstract: Flavonoids have been shown to modulate GLP-1 in obesity. GLP-1 induces some of its effects through the intestinal GLP-1 receptor (GLP-1R), though no data exist on how flavonoids affect this receptor. Here, we examine how a dose of grape seed proanthocyanidin extract (GSPE) with anti-obesity activity affects intestinal GLP-1R and analyze whether epigenetics play a role in the long-lasting effects of GSPE. We found that 10-day GSPE administration prior to the cafeteria diet upregulated GLP-1R mRNA in the ileum 17 weeks after the GSPE treatment. This was associated with a hypomethylation of the GLP-1R promoter near the region where the SP1 transcription factor binds. In the colon, the cafeteria diet upregulated GLP-1R without showing any GSPE effect. In conclusion, we have identified long-lasting GSPE effects on GLP-1R gene expression in the ileum that are partly mediated by hypomethylation at the gene promoter and may affect the SP1 binding factor.

Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 2218273X

Author's mail: ximena.terra@urv.cat raul.beltran@urv.cat montserrat.pinent@urv.cat mteresa.blay@urv.cat anna.ardevol@urv.cat

Author identifier: 0000-0003-1043-5844 0000-0001-9691-1906 0000-0003-3550-5378 0000-0002-6256-9847 0000-0003-0156-7538

Record's date: 2023-02-26

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2218-273X/9/12/865

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Biomolecules. 9 (12):

APA: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M.; Terra, Ximena; Ardeyol, Anna; Pinent, Mon (2019). Long-Lasting Effects of GSPE on Ileal GLP-1R Gene Expression Are Associated with a Hypomethylation of the GLP-1R Promoter in Female Wistar Rats. Biomolecules, 9(12), -. DOI: 10.3390/biom9120865

Article's DOI: 10.3390/biom9120865

Entity: Universitat Rovira i Virgili

Journal publication year: 2019

Publication Type: Journal Publications