Author, as appears in the article.: Rodríguez-Andrade E; Stchigel A; Guarro J; Cano-Lira J

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Cano Lira, José Francisco / Guarro Artigas, Josep / Stchigel Glikman, Alberto Miguel

Keywords: Yeasts Wine Taxonomy Taint Stoppers Spoilage Sparkling Sp nov. Ribosomal dna Phylogeny Kirschsteiniothelia Identification Fungi Cork taint Cava Biodiversity Bark Ascomycota stoppers spoilage sparkling fungi cork taint cava ascomycota

Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Filamentous fungi are rarely reported as responsible for spoiling wine. Cork taint was detected in sparkling wine; therefore, we investigated fungal contamination as a possible cause of organoleptic alteration. Spoiled wine was filtered and membranes were plated onto potato dextrose agar (PDA). The cork stoppers used for sealing bottles were cut and also plated onto PDA. Fungal strains were phenotypically characterized and molecularly identified by sequencing of a fragment of the 28S nrRNA gene (LSU) and (occasionally) by other additional molecular markers. Twentyseven strains were isolated and sixteen species were identified, all of them belonging to the phylum Ascomycota. The fungi isolated from wine were three species of Aspergillus section Nidulantes, a species of Penicillium section Exicaulis and Beauveria bassiana. Candida patagonica was isolated from both sort of samples, and the fungi isolated from cork stoppers were Altenaria alternata and Cladosporium cladosporioides. Surprisingly, most of the taxa recovered from the cork stoppers and/or wine were new to the science: a new genus (Dactylodendron) and seven new species belonging to the genera Cladophialophora, Dactylodendron, Kirschsteiniothelia, Rasamsonia, and Talaromyces. Future studies could let us know if these fungi would be able to produce compounds responsible for cork taint.

Thematic Areas: Virology Microbiology (medical) Microbiology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 20762607

Author's mail: josep.guarro@urv.cat jose.cano@urv.cat albertomiguel.stchigel@urv.cat

Author identifier: 0000-0002-7839-7568 0000-0003-4495-4394 0000-0003-3987-7996

Record's date: 2023-03-06

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2076-2607/8/1/12

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Microorganisms. 8 (1):

APA: Rodríguez-Andrade E; Stchigel A; Guarro J; Cano-Lira J (2020). Fungal diversity of deteriorated sparkling wine and cork stoppers in catalonia, Spain. Microorganisms, 8(1), -. DOI: 10.3390/microorganisms8010012

Article's DOI: 10.3390/microorganisms8010012

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications