Author, as appears in the article.: Oliva-Olivera, Wilfredo; Moreno-Indias, Isabel; Coin-Araguez, Leticia; Lhamyani, Said; Alcaide Torres, Juan; Fernandez-Veledo, Sonia; Vendrell, Joan; Camargo, Antonio; El Bekay, Rajaa; Jose Tinahones, Francisco;

Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques

URV's Author/s: Fernandez Veledo, Sonia / Vendrell Ortega, Juan José

Keywords: Tissue angiogenesis Stromal cells Progenitor cells Oxidative stress Mesenchymal stem-cells Macrophage infiltration Insulin-resistance Inflammation Improve postnatal neovascularization Endothelial-cells

Abstract: Background/Objectives Multiple studies suggest that hypoxia, together with inflammation, could be one of the phenomena involved in the onset and progression of obesity-related insulin resistance. In addition, dysfunction of adipose tissue in obese subjects with metabolic syndrome is associated with decreased angiogenesis. However, some subjects with a high body mass index do not develop metabolic abnormalities associated with obesity. The aim of the current study was to examine the neovascular properties of visceral adipose tissue-derived multipotent mesenchymal cells subjected to hypoxia (hypox-visASCs) from normal-weight subjects (Nw) and obese patients with metabolic syndrome (MS) and without metabolic syndrome (NonMS). Methods This was a 2-year study to enroll subjects who underwent bariatric surgery or cholecystectomy. Eight patients who underwent either bariatric surgery or cholecystectomy (27 patients) participated in the study. Visceral adipose tissue samples from Nw, MS and NonMS subjects were processed by enzymatic digestion. VisASCs cultured under hypoxic conditions were characterized by tubule formation assay, ELISA, flow cytometry, migration rate, and qRT-PCR, and the effects of visASCs-conditioned medium on survival and endothelial cell tubule formation were evaluated. Results Hypox-visASCs from NonMS subjects showed a greater capacity for tubule formation than hypox-visASCs from Nw and MS subjects. The lower percentage of CD140b(+)/CD44(+) and CD140b(+)/CD184(+) cells observed in hypox-visASCs from NonMS subjects compared to MS subjects was accompanied not only by a lower migration rate from the chemotactic effects of stromal cell derived factor 1 alpha, but also by lower levels of NOX5 mRNA expression. While the levels of monocyte chemoattractant protein 1 mRNA expressed by hypox-visASCs correlated positively with the body mass index and waist circumference of the subjects, the concentration of vascular endothelial growth factor present in hypox-visASC-conditioned culture medium decreased significantly with increasing plasma glucose. The survival rate and tubules formed by endothelial cells cultured in hypox-visASC-conditioned medium decreased significantly with increasing homeostasis model assessment to quantify insulin resistance. Conclusions Our results suggest that hypox-visASCs from NonMS subjects could promote healthy adipose tissue expansion, while hypox-visASCs from MS subjects appear to contribute to the decreased angiogenic potential and increased inflammation underlying adipose tissue dysfunction in obesity. Our results emphasize the importance of taking into account not only the BMI but also the metabolic profile of the subjects during the implementation of ASCs-based therapy to promote neovascularization.

Thematic Areas: Zootecnia / recursos pesqueiros Sociology Sociología Serviço social Saúde coletiva Química Psychology Psicología Planejamento urbano e regional / demografia Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Linguística e literatura Letras / linguística Interdisciplinary research in the social sciences Interdisciplinar Human geography and urban studies History & philosophy of science Historia Geografía Geociências General medicine General biochemistry,genetics and molecular biology General agricultural and biological sciences Farmacia Environmental studies Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Enfermagem Educação física Educação Economia Direito Demography Comunicação e informação Ciências sociais aplicadas i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência política e relações internacionais Ciência de alimentos Ciência da computação Biotecnología Biology Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Astronomia / física Arquitetura, urbanismo e design Archaeology Antropologia / arqueologia Anthropology Agricultural and biological sciences (miscellaneous) Administração, ciências contábeis e turismo Administração pública e de empresas, ciências contábeis e turismo

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 19326203

Author's mail: sonia.fernandez@urv.cat juanjose.vendrell@urv.cat

Author identifier: 0000-0003-2906-3788 0000-0002-6994-6115

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0188324

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Plos One. 12 (11): e0188324-

APA: Oliva-Olivera, Wilfredo; Moreno-Indias, Isabel; Coin-Araguez, Leticia; Lhamyani, Said; Alcaide Torres, Juan; Fernandez-Veledo, Sonia; Vendrell, Joan; (2017). Different response to hypoxia of adipose-derived multipotent cells from obese subjects with and without metabolic syndrome. Plos One, 12(11), e0188324-. DOI: 10.1371/journal.pone.0188324

Article's DOI: 10.1371/journal.pone.0188324

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

Publication Type: Journal Publications