Author, as appears in the article.: Perez, Laura M.; Bernal, Aurora; San Martin, Nuria; Lorenzo, Margarita; Fernandez-Veledo, Sonia; Galvez, Beatriz G.;

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Fernandez Veledo, Sonia

Keywords: Tumor-necrosis-factor Tissue Skeletal-muscle Insulin-resistance Hyperinsulinemia Glucose Free fatty-acids Differentiation Alpha Adipogenesis

Abstract: Adipose-derived stem cells (ASCs) are promising candidates for autologous cell-based regeneration therapies by virtue of their multilineage differentiation potential and immunogenicity; however, relatively little is known about their role in adipose tissue physiology and dysfunction. Here we evaluated whether ASCs isolated from nonobese and obese tissue differed in their metabolic characteristics and differentiation potential. During differentiation to mature adipocytes, mouse and human ASCs derived from nonobese tissues both increased their insulin sensitivity and inhibition of lipolysis, whereas obese-derived ASCs were insulin-resistant, showing impaired insulin-stimulated glucose uptake and resistance to the antilipolytic effect of insulin. Furthermore, obese-derived ABCs showed enhanced release of proinflammatory cytokines and impaired production of adiponectin. Interestingly, the delivery of cytosol from control ABCs into obese-derived ASCs using a lipid-based, protein-capture methodology restored insulin sensitivity on glucose and lipid metabolism and reversed the proinflammatory cytokine profile, in part due to the restoration of Lin28 protein levels. In conclusion, glucose and lipid metabolism as well as maturation of ABCs is truncated in an obese environment. The reversal of the altered pathways in obese cells by delivery of normal subcellular fractions offers a potential new tool for cell therapy.

Thematic Areas: Saúde coletiva Química Psicología Odontología Nutrição Medicina iii Medicina ii Medicina i Internal medicine Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Ciência da computação Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 00121797

Author's mail: sonia.fernandez@urv.cat

Author identifier: 0000-0003-2906-3788

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://diabetes.diabetesjournals.org/content/62/7/2368

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Diabetes. 62 (7): 2368-2379

APA: Perez, Laura M.; Bernal, Aurora; San Martin, Nuria; Lorenzo, Margarita; Fernandez-Veledo, Sonia; Galvez, Beatriz G.; (2013). Metabolic Rescue of Obese Adipose-Derived Stem Cells by Lin28/Let7 Pathway. Diabetes, 62(7), 2368-2379. DOI: 10.2337/db12-1220

Article's DOI: 10.2337/db12-1220

Entity: Universitat Rovira i Virgili

Journal publication year: 2013

Publication Type: Journal Publications