Author, as appears in the article.: Schreiber E; Garcia T; González N; Esplugas R; Sharma RP; Torrente M; Kumar V; Bovee T; Katsanou ES; Machera K; Domingo JL; Gómez M

Department: Enginyeria Química Psicologia

URV's Author/s: Domingo Roig, José Luis / Esplugas Borràs, Roser / Gómez Arnaiz, María Mercedes / Gonzalez Paradell, Nieves / Kumar, Vikas / Torrente Torné, Margarita

Keywords: Sexual-differentiation Reproductive development Nipple retention Mixtures Male rats Liquid-phase microextraction Linuron Flutamide Feminization effects Extraction sorbent Estrogenic compounds Endocrine disruptors Dienestrol Anti-androgens Anogenital distance Androgen receptor male rats linuron flutamide feminization effects dienestrol

Abstract: © 2020 The Authors Exposure to endocrine-disrupting compounds (EDCs) during pregnancy can result in negative health effects in later generations, including sex changes and feminization. The present study assessed the feminization effects on male offspring rats of three EDCs: Dienestrol (DIES), Linuron (LIN), and Flutamide (FLU). Sexually mature female rats were exposed from gestation day (GD) 6 until postnatal day (PND) 21 to: 0.37, 0.75, 1.5, 3.12 or 6.25 μg/kg/day of DIES, 1.5, 3, 6, 12.5, 25 or 50 mg/kg/day of LIN, 3.5, 6.7, 12.5, 25 or 50 mg/kg/day of FLU, and the following mixtures: FLU + DIES (mg/kg/day+μg/kg/day), 3.5 + 0.37, or 3.5 + 3, 25 + 0.37, or 25 + 3; FLU + LIN (mg/kg/day + mg/kg/day), 3.5 + 12.5, or 25 + 12.5; and DIES + LIN (μg/kg/day + mg/kg/day), 0.37 + 12.5, or 3 + 12.5. Anogenital distance (AGD), nipple retention (NR) and cryptorchidism were evaluated. FLU produced a decrease of AGD, an increase of NR, and an increase of cryptorchidism at the highest dose. None of these three endpoints were significantly affected by LIN or DIES treatments alone. Combinations of FLU + LIN and FLU + DIES increased NR, and decreased AGD, while DIES + LIN did not produce any effects in male pups. Results show that FLU is able to induce feminization in male pups, while binary combinations of LIN and DIES did not modify the effects produced by FLU.

Thematic Areas: Toxicology Saúde coletiva Química Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Food science & technology Food science Farmacia Ensino Engenharias iv Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 02786915

Author's mail: nieves.gonzalez@urv.cat vikas.kumar@urv.cat margarita.torrente@urv.cat joseluis.domingo@urv.cat

Author identifier: 0000-0003-4134-016X 0000-0002-9795-5967 0000-0002-8901-6345 0000-0001-6647-9470

Record's date: 2023-02-22

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.sciencedirect.com/science/article/pii/S0278691520301447

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Food And Chemical Toxicology. 139 (111256): 111256-

APA: Schreiber E; Garcia T; González N; Esplugas R; Sharma RP; Torrente M; Kumar V; Bovee T; Katsanou ES; Machera K; Domingo JL; Gómez M (2020). Maternal exposure to mixtures of dienestrol, linuron and flutamide. Part I: Feminization effects on male rat offspring. Food And Chemical Toxicology, 139(111256), 111256-. DOI: 10.1016/j.fct.2020.111256

Article's DOI: 10.1016/j.fct.2020.111256

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications