Author, as appears in the article.: Gonzalez-Quilen, Carlos; Grau-Bove, Carme; Jorba-Martin, Rosa; Caro-Tarrago, Aleidis; Pinent, Montserrat; Ardevol, Anna; Beltran-Debon, Raul; Terra, Ximena; Blay, M. Teresa;

Department: Bioquímica i Biotecnologia

URV's Author/s: Ardèvol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / Jorba Martin, Rosa Maria / Pinent Armengol, Montserrat / Terra Barbadora, Ximena

Keywords: Ussing chamber Signal-transduction pathways Release Rats Procyanidin Permeability Macrophages Inflammation Human colon Flavonoid Flavanols Extract Disease Cytokines Colitis procyanidin permeability inflammation human colon flavonoid

Abstract: Purpose Anti-inflammatory and barrier-protective properties have been attributed to proanthocyanidins in the context of intestinal dysfunction, however little information is available about the impact of these phytochemicals on intestinal barrier integrity and immune response in the human. Here we assessed the putative protective properties of a grape-seed proanthocyanidin extract (GSPE) against dextran sodium sulfate (DSS)-induced acute dysfunction of the human colon in an Ussing chamber system. Methods Human proximal and distal colon tissues from colectomized patients were submitted ex vivo for a 30-min preventive GSPE treatment (50 or 200 mu g mL(-1)) followed by 1-h incubation with DSS (12% w v(-1)). Transepithelial electrical resistance (TEER), permeation of a fluorescently-labeled dextran (FD4) and proinflammatory cytokine release [tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta] of colonic tissues were determined. Results DSS reduced TEER (45-52%) in both the proximal and distal colon; however, significant increments in FD4 permeation (fourfold) and TNF-alpha release (61%) were observed only in the proximal colon. The preventive GSPE treatment decreased DSS-induced TEER loss (20-32%), FD4 permeation (66-73%) and TNF-alpha release (22-33%) of the proximal colon dose-dependently. The distal colon was not responsive to the preventive treatment but showed a reduction in IL-1 beta release below basal levels with the highest GSPE concentration. Conclusions Our results demonstrate potential preventive effects of GSPE on human colon dysfunction. Further studies are required to test whether administering GSPE could be a complementary therapeutic approach in colonic dysfunction associated with metabolic disorders and inflammatory bowel disease.

Thematic Areas: Serviço social Saúde coletiva Química Nutrition and dietetics Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1436-6207

Author's mail: rosamaria.jorba@urv.cat ximena.terra@urv.cat raul.beltran@urv.cat montserrat.pinent@urv.cat mteresa.blay@urv.cat anna.ardevol@urv.cat

Author identifier: 0000-0003-3307-4340 0000-0003-1043-5844 0000-0001-9691-1906 0000-0003-3550-5378 0000-0002-6256-9847 0000-0003-0156-7538

Record's date: 2023-02-26

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://link.springer.com/article/10.1007%2Fs00394-020-02222-3

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: European Journal Of Nutrition. 60 (1): 79-88

APA: Gonzalez-Quilen, Carlos; Grau-Bove, Carme; Jorba-Martin, Rosa; Caro-Tarrago, Aleidis; Pinent, Montserrat; Ardevol, Anna; Beltran-Debon, Raul; Terra, X (2021). Protective properties of grape-seed proanthocyanidins in human ex vivo acute colonic dysfunction induced by dextran sodium sulfate. European Journal Of Nutrition, 60(1), 79-88. DOI: 10.1007/s00394-020-02222-3

Article's DOI: 10.1007/s00394-020-02222-3

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications