Author, as appears in the article.: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, Josep; Diogene, Jorge; O'Sullivan, Ciara K.; Alcaraz, Carles; Campas, Monica;

Department: Enginyeria Química

URV's Author/s: CAMPAS HOMS, MONICA / O'SULLIVAN, CIARA KATHLEEN

Keywords: Islands ne atlantic toxins sandwich immunoassay ocean monoclonal-antibodies immunoassay stick test gambierdiscus fish ciguatera 1st report

Abstract: The importance of ciguatoxins (CTXs) in seafood safety and their emerging occurrence in locations far away from tropical areas highlight the need for simple and low-cost methods for the sensitive and rapid detection of these potent marine toxins to protect seafood consumers. Herein, an electrochemical immunosensor for the detection of CTXs is presented. A sandwich configuration is proposed, using magnetic beads (MBs) as immobilization supports for two capture antibodies, with their combination facilitating the detection of CTX1B, CTX3C, 54-deoxyCTX1B, and 51-hydroxyCTX3C. PoIyHRP-streptavidin is used for the detection of the biotinylated detector antibody. Experimental conditions are first optimized using colorimetry, and these conditions are subsequently used for electrochemical detection on electrode arrays. Limits of detection at the pg/mL level are achieved for CTX1B and 5 1-hydroxyCTX3C. The applicability of the immunosensor to the analysis of fish samples is demonstrated, attaining detection of CTX1B at contents as low as 0.01 mu g/kg and providing results in correlation with those obtained using mouse bioassay (MBA) and cell-based assay (CBA), and confirmed by liquid chromatography coupled to high-resolution mass spectrometry (LC-ESI-HRMS). This user-friendly bioanalytical tool for the rapid detection of CTXs can be used to mitigate ciguatera risk and contribute to the protection of consumer health.

Thematic Areas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 0003-2700

Author's mail: monica.campas@urv.cat

Author identifier: 0000-0002-1220-7100

Last page: 4865

Record's date: 2022-11-19

Journal volume: 92

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b04499

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Analytical Chemistry. 92 (7): 4858-4865

APA: Leonardo, Sandra; Gaiani, Greta; Tsumuraya, Takeshi; Hirama, Masahiro; Turquet, Jean; Sagrista, Nuria; Rambla-Alegre, Maria; Flores, Cintia; Caixach, (2020). Addressing the Analytical Challenges for the Detection of Ciguatoxins Using an Electrochemical Biosensor. Analytical Chemistry, 92(7), 4858-4865. DOI: 10.1021/acs.analchem.9b04499

Article's DOI: 10.1021/acs.analchem.9b04499

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

First page: 4858

Publication Type: Journal Publications