Author, as appears in the article.: Marsillach J; Aragonès G; Mackness B; Mackness M; Rull A; Beltrán-Debón R; Pedro-Botet J; Alonso-Villaverde C; Joven J; Camps J

Department: Medicina i Cirurgia

URV's Author/s: Aragonès Bargalló, Gerard / Beltrán Debón, Raúl Alejandro / Camps Andreu, Jorge / Joven Maried, Jorge / RULL AIXA, ANNA

Abstract: © 2010 Marsillach et al; licensee BioMed Central Ltd. Background: Paraoxonase-1 (PON1), a lactonase synthesized by the liver, circulates in blood bound to high-density lipoproteins (HDL). This enzyme is thought to degrade oxidized phospholipids and play an important role in the organism's antioxidant and anti-inflammatory system. Chronic liver diseases are characterized by decreased serum PON1 activity. The aim of the present study was to investigate the compositional changes in HDL that could influence PON1 activity in liver impairment. Methods: The study was performed in samples from five patients with advanced liver cirrhosis and with preserved renal function, chosen on the basis of having low serum PON1 activity and high serum PON1 concentration. As a control group, we accessed five healthy volunteers from among our hospital staff. Lipid and protein compositional analysis of lipoprotein particles were done by high-performance liquid chromatography, gel electrophoresis, and Western-Blot. Results: HDL particles from cirrhotic patients had an increased phospholipid content that was inversely correlated to PON1 activity. The HDL particles contained high levels of PON1 that corresponded, in part, to an immunoreactive protein of high molecular weight (55 kDa) not present in control subjects. This protein was identified as glycosylated PON1 and was also present in biopsies from patients with steatosis and from rats with CCl4-induced hepatic impairment. These changes were associated with an increased plasma concentration of markers of oxidative stress, inflammation and fibrogenesis. Conclusion: Abnormalities in the composition of lipids and proteins of HDL particles, including PON1 glycosylation, are associated with the decrease in serum PON1 activity in patients with liver disease. These alterations may adversely affect the protective role of HDL against oxidative stress and inflammation in these patients.

Thematic Areas: Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Ensino Enfermagem Endocrinology, diabetes and metabolism Endocrinology Educação física Clinical biochemistry Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Biochemistry (medical) Biochemistry & molecular biology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1476-511X

Author's mail: jorge.camps@urv.cat gerard.aragones@urv.cat raul.beltran@urv.cat jorge.joven@urv.cat

Author identifier: 0000-0002-3165-3640 0000-0001-9691-1906 0000-0003-2749-4541

Record's date: 2023-02-19

Journal volume: 9

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://lipidworld.biomedcentral.com/articles/10.1186/1476-511X-9-46

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Lipids In Health And Disease. 9 (1): 46-

APA: Marsillach J; Aragonès G; Mackness B; Mackness M; Rull A; Beltrán-Debón R; Pedro-Botet J; Alonso-Villaverde C; Joven J; Camps J (2010). Decreased paraoxonase-1 activity is associated with alterations of high-density lipoprotein particles in chronic liver impairment. Lipids In Health And Disease, 9(1), 46-. DOI: 10.1186/1476-511X-9-46

Article's DOI: 10.1186/1476-511X-9-46

Entity: Universitat Rovira i Virgili

Journal publication year: 2010

Publication Type: Journal Publications