Author, as appears in the article.: Aragonès G; Saavedra P; Heras M; Cabré A; Girona J; Masana L

Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques

URV's Author/s: ARAGONÈS BARGALLÓ, GEMMA / Girona Tell, Josefa / HERAS IBAÑEZ, MERCEDES / Masana Marín, Luis / SAAVEDRA GARCIA, PAULA

Keywords: Resistance Plasma fatty-acid-binding-protein-4 Nitric oxide (no) Metabolic syndrome Mechanisms Insulin-signalling pathway Insulin Heart Fatty acid-binding protein 4 (fabp4) Expression Endothelium Endothelial nitric oxide synthase (enos) Endothelial dysfunction Dysfunction Diabetes Coronary-artery-disease Atherosclerosis Adipose-tissue depots

Abstract: Background: Recent studies have shown that fatty acid-binding protein 4 (FABP4) plasma levels are associated with impaired endothelial function in type 2 diabetes (T2D). In this work, we analysed the effect of FABP4 on the insulin-mediated nitric oxide (NO) production by endothelial cells in vitro.Methods: In human umbilical vascular endothelial cells (HUVECs), we measured the effects of FABP4 on the insulin-mediated endothelial nitric oxide synthase (eNOS) expression and activation and on NO production. We also explored the impact of exogenous FABP4 on the insulin-signalling pathway (insulin receptor substrate 1 (IRS1) and Akt).Results: We found that eNOS expression and activation and NO production are significantly inhibited by exogenous FABP4 in HUVECs. FABP4 induced an alteration of the insulin-mediated eNOS pathway by inhibiting IRS1 and Akt activation. These results suggest that FABP4 induces endothelial dysfunction by inhibiting the activation of the insulin-signalling pathway resulting in decreased eNOS activation and NO production.Conclusion: These findings provide a mechanistic linkage between FABP4 and impaired endothelial function in diabetes, which leads to an increased cardiovascular risk. © 2012 Aragonès et al.; licensee BioMed Central Ltd.

Thematic Areas: Saúde coletiva Medicina ii Medicina i Internal medicine Interdisciplinar Farmacia Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Cardiology and cardiovascular medicine Cardiac & cardiovascular systems Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14752840

Author's mail: josefa.girona@urv.cat luis.masana@urv.cat josefa.girona@urv.cat

Author identifier: 0000-0002-6267-8779 0000-0002-0789-4954 0000-0002-6267-8779

Record's date: 2023-05-20

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://cardiab.biomedcentral.com/articles/10.1186/1475-2840-11-72

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Cardiovascular Diabetology. 11

APA: Aragonès G; Saavedra P; Heras M; Cabré A; Girona J; Masana L (2012). Fatty acid-binding protein 4 impairs the insulin-dependent nitric oxide pathway in vascular endothelial cells. Cardiovascular Diabetology, 11(), -. DOI: 10.1186/1475-2840-11-72

Article's DOI: 10.1186/1475-2840-11-72

Entity: Universitat Rovira i Virgili

Journal publication year: 2012

Publication Type: Journal Publications