Author, as appears in the article.: Callau C; Lejeune M; Korzynska A; García M; Bueno G; Bosch R; Jaén J; Orero G; Salvadó T; López C

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Lejeune, Marylène Marie / Lopez Pablo, Carlos

Keywords: Tumour markers Tissue array analysis Immunohistochemistry Image processing Breast neoplasms Algorithms

Abstract: © 2015 Callau et al. Background: Digital image (DI) analysis avoids visual subjectivity in interpreting immunohistochemical stains and provides more reproducible results. An automated procedure consisting of two variant methods for quantifying the cytokeratin-19 (CK19) marker in breast cancer tissues is presented. Methods: The first method (A) excludes the holes inside selected CK19 stained areas, and the second (B) includes them. 93 DIs scanned from complete cylinders of tissue microarrays were evaluated visually by two pathologists and by the automated procedures. Results and conclusions: There was good concordance between the two automated methods, both of which tended to identify a smaller CK19-positive area than did the pathologists. The results obtained with method B were more similar to those of the pathologists; probably because it takes into account the entire positive tumoural area, including the holes. However, the pathologists overestimated the positive area of CK19. Further studies are needed to confirm the utility of this automated procedure in prognostic studies.

Thematic Areas: Saúde coletiva Radiology, nuclear medicine and imaging Radiological and ultrasound technology Psicología Odontología Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar Engineering, biomedical Engenharias iv Engenharias iii Educação física Ciências biológicas ii Ciências biológicas i Ciência da computação Biotecnología Biomedical engineering Biomaterials Astronomia / física Administração pública e de empresas, ciências contábeis e turismo

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1475925X

Author's mail: marylenemarie.lejeune@urv.cat carlos.lopez@urv.cat

Author identifier: 0000-0001-8441-9404 0000-0003-1248-3065

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://biomedical-engineering-online.biomedcentral.com/articles/10.1186/1475-925X-14-S2-S2

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Biomedical Engineering Online. 14 (2): S2-

APA: Callau C; Lejeune M; Korzynska A; García M; Bueno G; Bosch R; Jaén J; Orero G; Salvadó T; López C (2015). Evaluation of cytokeratin-19 in breast cancer tissue samples: A comparison of automatic and manual evaluations of scanned tissue microarray cylinders. Biomedical Engineering Online, 14(2), S2-. DOI: 10.1186/1475-925X-14-S2-S2

Article's DOI: 10.1186/1475-925X-14-S2-S2

Entity: Universitat Rovira i Virgili

Journal publication year: 2015

Publication Type: Journal Publications