Author, as appears in the article.: Siqueira J; Sutton D; Gené J; García D; Guevara-Suarez M; Decock C; Wiederhold N; Guarro J

Department: Ciències Mèdiques Bàsiques

URV's Author/s: García Sánchez, Dania / Gené Díaz, Josepa / Guarro Artigas, Josep / ZEN SIQUEIRA, JOAO PAULO

Keywords: Rhinosinusitis Molecular characterization Infection Identification Epitypification Commune sinusitis Brain-abscess Basidiomycete Antifungal susceptibility Allergic bronchopulmonary mycosis

Abstract: Copyright © 2016, American Society for Microbiology. All Rights Reserved. Schizophyllum is an important genus of basidiomycetes that, apart from being of genetic and biotechnological interest, is also reported to be a plant and animal pathogen. Schizophyllum commune is the best-known species and the only one reported from clinical specimens thus far, being recovered mainly from the respiratory tract. The aim of this study was to determine the species diversity of 23 clinical isolates of Schizophyllum from the United States using multilocus phylogenetic analysis and their in vitro susceptibilities to six drugs. The markers used for sequencing were the internal transcribed spacer (ITS), a portion of the nuclear large subunit (LSU) of ribosomal DNA, the RNA polymerase II second-largest subunit (RPB2), and the translation elongation factor 1α (EF-1α) gene. The analyses revealed that 22 of the clinical isolates were in the Schizophyllum radiatum clade with high support values and 1 isolate was in the S. commune clade. This is the first report of this species in clinical samples. The two species mentioned above showed very similar morphological features in culture (i.e., white, cottony, unsporulated colonies composed of hyphae with clamp connections), making morphological discrimination between the two impossible. An epitype is designed for S. radiatum, and its sequences have been deposited in GenBank. The antifungal that showed the greatest in vitro activity against the strains tested was shown to be amphotericin B. In general, the strains of S. radiatum showed higher MICs than S. commune.

Research group: Unitat de Micologia i Microbiologia Ambiental

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Ensino Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Ciência da computação Biotecnología Biodiversidade

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 00951137

Author's mail: josep.guarro@urv.cat josepa.gene@urv.cat dania.garcias@urv.cat

Author identifier: 0000-0002-7839-7568 0000-0001-6195-9299 0000-0003-2397-1646

Record's date: 2023-05-21

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://jcm.asm.org/content/54/10/2491.short

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Journal Of Clinical Microbiology. 54 (10): 2491-2497

APA: Siqueira J; Sutton D; Gené J; García D; Guevara-Suarez M; Decock C; Wiederhold N; Guarro J (2016). Schizophyllum radiatum, an emerging fungus from human respiratory tract. Journal Of Clinical Microbiology, 54(10), 2491-2497. DOI: 10.1128/JCM.01170-16

Article's DOI: 10.1128/JCM.01170-16

Entity: Universitat Rovira i Virgili

Journal publication year: 2016

Publication Type: Journal Publications