Author, as appears in the article.: Nadal L; Garcia N; Hurtado E; Simó A; Tomàs M; Lanuza M; Cilleros V; Tomàs J

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Cilleros Mañé, Víctor / Garcia Sancho, Maria de les Neus / HURTADO CABALLERO, ERICA / Lanuza Escolano, María Angel / NADAL MAGRIÑÀ, LAURA / SIMÓ OLLÉ, ANNA / Tomás Ferré, José Maria / Tomas Marginet, Marta

Keywords: Trkb receptor Synapse elimination Presynaptic muscarinic acetylcholine receptors Neuromuscular junction Motor nerve terminal Cholinergic synapses trkb receptor presynaptic muscarinic acetylcholine receptors neuromuscular junction motor nerve terminal cholinergic synapses

Abstract: © 2017 Nadal, Garcia, Hurtado, Simó, Tomàs, Lanuza, Cilleros and Tomàs. The development of the nervous system involves the overproduction of synapses but connectivity is refined by Hebbian activity-dependent axonal competition. The newborn skeletal muscle fibers are polyinnervated but, at the end of the competition process, some days later, become innervated by a single axon. We used quantitative confocal imaging of the autofluorescent axons from transgenic B6.Cg-Tg (Thy1-YFP)16 Jrs/J mice to investigate the possible cooperation of the muscarinic autoreceptors (mAChR, M1-, M2- and M4-subtypes) and the tyrosine kinase B (TrkB) receptor in the control of axonal elimination after the mice Levator auris longus (LAL) muscle had been exposed to several selective antagonist of the corresponding receptor pathways in vivo. Our previous results show that M1, M2 and TrkB signaling individually increase axonal loss rate around P9. Here we show that although the M1 and TrkB receptors cooperate and add their respective individual effects to increase axonal elimination rate even more, the effect of the M2 receptor is largely independent of both M1 and TrkB receptors. Thus both, cooperative and non-cooperative signaling mechanisms contribute to developmental synapse elimination.

Thematic Areas: Saúde coletiva Química Psicología Nutrição Neurosciences Medicina veterinaria Medicina ii Medicina i Interdisciplinar Geriatrics & gerontology Engenharias iv Educação física Cognitive neuroscience Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biodiversidade Aging

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 16634365

Author's mail: victor.cilleros@urv.cat marta.tomas@urv.cat mariaangel.lanuza@urv.cat josepmaria.tomas@urv.cat victor.cilleros@urv.cat

Author identifier: 0000-0001-5690-9932 0000-0002-4151-1697 0000-0003-4795-4103 0000-0002-0406-0006 0000-0001-5690-9932

Record's date: 2023-09-02

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.frontiersin.org/articles/10.3389/fnagi.2017.00024/full

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Frontiers In Aging Neuroscience. 9 (FEB): 24-

APA: Nadal L; Garcia N; Hurtado E; Simó A; Tomàs M; Lanuza M; Cilleros V; Tomàs J (2017). Presynaptic muscarinic acetylcholine receptors and TrkB receptor cooperate in the elimination of redundant motor nerve terminals during development. Frontiers In Aging Neuroscience, 9(FEB), 24-. DOI: 10.3389/fnagi.2017.00024

Article's DOI: 10.3389/fnagi.2017.00024

Entity: Universitat Rovira i Virgili

Journal publication year: 2017

Publication Type: Journal Publications