Author, as appears in the article.: Jiménez-Lamana J; Godin S; Aragonès G; Bladé C; Szpunar J; Łobinski R

Department: Bioquímica i Biotecnologia

URV's Author/s: Aragonès Bargalló, Gerard / BLADÉ SEGARRA, MARIA CINTA

Keywords: Toxicity Skin Silver nanoparticles Protein induction Nickel nanoparticles Molecular-mechanisms Mass-spectrometry Human keratinocytes High resolution mass spectrometry Cytotoxicity Culture-medium Chemical carcinogens Cells Bioaccumulation nickel nanoparticles human keratinocytes high resolution mass spectrometry cytotoxicity

Abstract: © 2020 by the authors. Although nickel allergy and carcinogenicity are well known, their molecular mechanisms are still uncertain, thus demanding studies at the molecular level. The nickel carcinogenicity is known to be dependent on the chemical form of nickel, since only certain nickel compounds can enter the cell. This study investigates, for the first time, the cytotoxicity, cellular uptake, and molecular targets of nickel nanoparticles (NiNPs) in human skin cells in comparison with other chemical forms of nickel. The dose-response curve that was obtained for NiNPs in the cytotoxicity assays showed a linear behavior typical of genotoxic carcinogens. The exposure of keratinocytes to NiNPs leads to the release of Ni2+ ions and its accumulation in the cytosol. A 6 kDa nickel-binding molecule was found to be synthesized by cells exposed to NiNPs at a dose corresponding to medium mortality. This molecule was identified to be tumor-related p63-regulated gene 1 protein.

Thematic Areas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 2079-4991

Author's mail: gerard.aragones@urv.cat

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2079-4991/10/5/992

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Nanomaterials. 10 (5):

APA: Jiménez-Lamana J; Godin S; Aragonès G; Bladé C; Szpunar J; Łobinski R (2020). Nickel nanoparticles induce the synthesis of a tumor-related polypeptide in human epidermal keratinocytes. Nanomaterials, 10(5), -. DOI: 10.3390/nano10050992

Article's DOI: 10.3390/nano10050992

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications