Author, as appears in the article.: Auguet T; Bertran L; Binetti J; Aguilar C; Martínez S; Sabench F; Lopez-Dupla JM; Porras JA; Riesco D; Del Castillo D; Richart C

Department: Medicina i Cirurgia

URV's Author/s: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Bertran Ramos, Laia / Binetti, Jessica Angela / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima

Keywords: Toll-like receptors Tnf-alpha Pathogenesis Oxidative stress Nonalcoholic steatohepatitis Nonalcoholic fatty liver disease Natural-history Morbid obesity Insulin-resistance Inflammation Fibrosis Fatty liver-disease Cytokines Adipokines nonalcoholic steatohepatitis nonalcoholic fatty liver disease morbid obesity cytokines

Abstract: The progression of nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) is linked to systemic inflammation. Currently, two of the aspects that need further investigation are diagnosis and treatment of NASH. In this sense, the aim of this study was to assess the relationship between circulating levels of cytokines, hepatic expression of toll-like receptors (TLRs), and degrees of NAFLD, and to investigate whether these levels could serve as noninvasive biomarkers of NASH. The present study assessed plasma levels of cytokines in 29 normal-weight women and 82 women with morbid obesity (MO) (subclassified: normal liver (n = 29), simple steatosis (n = 32), and NASH (n = 21)). We used enzyme-linked immunosorbent assays (ELISAs) to quantify cytokine and TLR4 levels and RTqPCR to assess TLRs hepatic expression. IL-1β, IL-8, IL-10, TNF-α, tPAI-1, and MCP-1 levels were increased, and adiponectin levels were decreased in women with MO. IL-8 was significantly higher in MO with NASH than in NL. To sum up, high levels of IL-8 were associated with the diagnosis of NASH in a cohort of women with morbid obesity. Moreover, a positive correlation between TLR2 hepatic expression and IL-8 circulating levels was found.

Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 14220067

Author's mail: joseantonio.porras@urv.cat danieldel.castillo@urv.cat mariasalome.martinez@urv.cat cristobalmanuel.richart@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat fatima.sabench@urv.cat laia.bertran@urv.cat jessicaangela.binetti@estudiants.urv.cat

Author identifier: 0000-0001-6418-1822 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X 0000-0002-9262-8756 0000-0001-9052-1368 0000-0001-9660-7752

Record's date: 2023-06-24

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/1422-0067/21/11/4189

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: International Journal Of Molecular Sciences. 21 (11): 1-15

APA: Auguet T; Bertran L; Binetti J; Aguilar C; Martínez S; Sabench F; Lopez-Dupla JM; Porras JA; Riesco D; Del Castillo D; Richart C (2020). Relationship between IL-8 Circulating Levels and TLR2 Hepatic Expression in Women with Morbid Obesity and Nonalcoholic Steatohepatitis. International Journal Of Molecular Sciences, 21(11), 1-15. DOI: 10.3390/ijms21114189

Article's DOI: 10.3390/ijms21114189

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications