Author, as appears in the article.: Mas-Capdevila A; Iglesias-Carres L; Arola-Arnal A; Aragonès G; Muguerza B; Bravo FI

Department: Bioquímica i Biotecnologia

URV's Author/s: Aragonès Bargalló, Gerard / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Muguerza Marquínez, Maria Begoña

Keywords: Uhplc-ms/ms Uhplc-ms Uhplc-hrms Proteins Nitric oxide Naloxone Ms Caco-2 cells Blood-pressure Blood pressure Bioavailability Bioactive peptides Alpha-lactorphin uhplc-ms/ms uhplc-hrms naloxone caco-2 cells blood pressure bioactive peptides

Abstract: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption.

Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 2218-273X

Author's mail: gerard.aragones@urv.cat franciscaisabel.bravo@urv.cat begona.muguerza@urv.cat anna.arola@urv.cat

Author identifier: 0000-0002-6468-3088 0000-0001-7384-8588 0000-0001-6529-1345

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2218-273X/10/7/992

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Biomolecules. 10 (7): 1-12

APA: Mas-Capdevila A; Iglesias-Carres L; Arola-Arnal A; Aragonès G; Muguerza B; Bravo FI (2020). Implication of opioid receptors in the antihypertensive effect of a novel chicken foot-derived peptide. Biomolecules, 10(7), 1-12. DOI: 10.3390/biom10070992

Article's DOI: 10.3390/biom10070992

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications