Author, as appears in the article.: Rodríguez-Calvo R; Samino S; Girona J; Martínez-Micaelo N; Ràfols P; García-Altares M; Guaita-Esteruelas S; Junza A; Heras M; Yanes O; Correig X; Masana L
Department: Enginyeria Electrònica, Elèctrica i Automàtica Medicina i Cirurgia
URV's Author/s: Correig Blanchar, Francesc Xavier / Garcia-Altares Pérez, Maria / Girona Tell, Josefa / GUAITA ESTERUELAS, SANDRA / HERAS IBAÑEZ, MERCEDES / Junza Martínez, Alexandra / Martínez Micaelo, Nieves Beatriz / Masana Marín, Luis / Ràfols Soler, Pere / SAMINO GENÉ, SARA / Yanes Torrado, Óscar
Keywords: Stress Steatosis Steatohepatitis Pathogenesis Nafld Liver triglycerides Lipidome Insulin-resistance Inhibition Inflammation Fabp4 Endoplasmic-reticulum De-novo lipogenesis Alpha Adipokines Acid-binding protein nafld lipidome inflammation fabp4 adipokines
Abstract: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. An imbalance between hepatic fatty acid uptake and removal results in ectopic fat accumulation, which leads to non-alcoholic fatty liver disease (NAFLD). The amount and type of accumulated triglycerides seem to play roles in NAFLD progression; however, a complete understanding of how triglycerides contribute to NAFLD evolution is lacking. Our aim was to evaluate triglyceride accumulation in NAFLD in a murine model and its associations with molecular mechanisms involved in liver damage and adipose tissue-liver cross talk by employing lipidomic and molecular imaging techniques. C57BL/6J mice fed a high-fat diet (HFD) for 12 weeks were used as a NAFLD model. Standard-diet (STD)-fed animals were used as controls. Standard liver pathology was assessed using conventional techniques. The liver lipidome was analyzed by liquid chromatography–mass spectrometry (LC–MS) and laser desorption/ionization–mass spectrometry (LDI–MS) tissue imaging. Liver triglycerides were identified by MS/MS. The transcriptome of genes involved in intracellular lipid metabolism and inflammation was assessed by RT-PCR. Plasma leptin, resistin, adiponectin, and FABP4 levels were determined using commercial kits. HFD-fed mice displayed increased liver lipid content. LC–MS analyses identified 14 triglyceride types that were upregulated in livers from HFD-fed animals. Among these 14 types, 10 were identified in liver cross sections by LDI–MS tissue imaging. The accumulation of these triglycerides was associated with the upregulation of lipogenesis and inflammatory genes and the downregulation of β-oxidation genes. Interestingly, the levels of plasma FABP4, but not of other adipokines, were positively associated with 8 of these triglycerides in HFD-fed mice but not in STD-fed mice. Our findings suggest a putative role of FABP4 in the liver-adipose tissue cross talk in NAFLD.
Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2218273X
Author's mail: xavier.correig@urv.cat maria.garcia-altares@urv.cat alexandra.junza@urv.cat josefa.girona@urv.cat oscar.yanes@urv.cat neus.martinez@urv.cat pere.rafols@urv.cat luis.masana@urv.cat josefa.girona@urv.cat
Author identifier: 0000-0002-6902-3054 0000-0001-7205-0419 0000-0002-6267-8779 0000-0003-3695-7157 0000-0002-9240-4058 0000-0002-0789-4954 0000-0002-6267-8779
Record's date: 2023-02-27
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2218-273X/10/9/1275
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Biomolecules. 10 (9): 1-15
APA: Rodríguez-Calvo R; Samino S; Girona J; Martínez-Micaelo N; Ràfols P; García-Altares M; Guaita-Esteruelas S; Junza A; Heras M; Yanes O; Correig X; Masa (2020). Hepatic lipidomics and molecular imaging in a murine non-alcoholic fatty liver disease model: Insights into molecular mechanisms. Biomolecules, 10(9), 1-15. DOI: 10.3390/biom10091275
Article's DOI: 10.3390/biom10091275
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications