Author, as appears in the article.: Cedo, Lidia; Fernandez-Castillejo, Sara; Rubio, Laura; Metso, Jari; Santos, David; Munoz-Aguayo, Daniel; Rivas-Urbina, Andrea; Tondo, Mireia; Mendez-Lara, Karen Alejandra; Farras, Marta; Jauhiainen, Matti; Motilva, Maria-Jose; Fito, Montserrat; Blanco-Vaca, Francisco; Sola, Rosa; Escola-Gil, Joan Carles;

Department: Medicina i Cirurgia

URV's Author/s: FERNÁNDEZ CASTILLEJO, SARA / Solà Alberich, Rosa Maria

Keywords: Virgin olive oil Risk-factors Reverse cholesterol transport Polyphenols Phenolic compounds Mice vohf project Increases Improves Humans Hdl Expression Efflux Consumption Antioxidant

Abstract: The intake of olive oil (OO) enriched with phenolic compounds (PCs) promotes ex vivo HDL-mediated macrophage cholesterol efflux in humans. We aimed to determine the effects of PC-enriched virgin OO on reverse cholesterol transport (RevCT) from macrophages to feces in vivo. Female C57BL/6 mice were given intragastric doses of refined OO (ROO) and a functional unrefined virgin OO enriched with its own PC (FVOO) for 14 days. Our experiments included two independent groups of mice that received intragastric doses of the phenolic extract (PE) used to prepare the FVOO and the vehicle solution (saline), as control, for 14 days. FVOO intake led to a significant increase in serum HDL cholesterol and its ability to induce macrophage cholesterol efflux in vitro when compared with ROO group. This was concomitant with the enhanced macrophage-derived [H-3]cholesterol transport to feces in vivo. PE intakeper sealso increased HDL cholesterol levels and significantly promoted in vivo macrophage-to-feces RevCT rate when compared with saline group. PE upregulated the expression of the main macrophage transporter involved in macrophage cholesterol efflux, theATP binding cassettea1. Our data provide direct evidence of the crucial role of OO PCs in the induction of macrophage-specific RevCT in vivo.

Thematic Areas: Pharmacology & pharmacy Medicine, research & experimental Medicine (miscellaneous) General biochemistry,genetics and molecular biology Ciencias sociales Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Biochemistry & molecular biology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: rosa.sola@urv.cat

Author identifier: 0000-0002-8359-235X

Record's date: 2023-04-02

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2227-9059/8/8/266

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Biomedicines. 8 (8):

APA: Cedo, Lidia; Fernandez-Castillejo, Sara; Rubio, Laura; Metso, Jari; Santos, David; Munoz-Aguayo, Daniel; Rivas-Urbina, Andrea; Tondo, Mireia; Mendez-L (2020). Phenol-Enriched Virgin Olive Oil Promotes Macrophage-Specific Reverse Cholesterol Transport In Vivo. Biomedicines, 8(8), -. DOI: 10.3390/biomedicines8080266

Article's DOI: 10.3390/biomedicines8080266

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications