Author, as appears in the article.: Castillo-Castañeda A; Cañas-Duarte SJ; Guevara-Suarez M; Guarro J; Restrepo S; Celis Ramírez AM

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Guarro Artigas, Josep

Keywords: Transcriptional changes Rna-seq Posaconazole Fusaria Amphotericin b.

Abstract: © 2020 The Authors. Some species of fusaria are well-known pathogens of humans, animals and plants. Fusarium oxysporum and Neocosmospora solani (formerly Fusarium solani) cause human infections that range from onychomycosis or keratitis to severe disseminated infections. In general, these infections are difficult to treat due to poor therapeutic responses in immunocompromised patients. Despite that, little is known about the molecular mechanisms and transcriptional changes responsible for the antifungal resistance in fusaria. To shed light on the transcriptional response to antifungals, we carried out the first reported high-throughput RNA-seq analysis for F. oxysporum and N. solani that had been exposed to amphotericin B (AMB) and posaconazole (PSC). We detected significant differences between the transcriptional profiles of the two species and we found that some oxidation-reduction, metabolic, cellular and transport processes were regulated differentially by both fungi. The same was found with several genes from the ergosterol synthesis, efflux pumps, oxidative stress response and membrane biosynthesis pathways. A significant up-regulation of the C-22 sterol desaturase (ERG5), the sterol 24-C-methyltransferase (ERG6) gene, the glutathione S-transferase (GST) gene and of several members of the major facilitator superfamily (MSF) was demonstrated in this study after treating F. oxysporum with AMB. These results offer a good overview of transcriptional changes after exposure to com-monly used antifungals, highlights the genes that are related to resistance mechanisms of these fungi, which will be a valuable tool for identifying causes of failure of treatments.

Thematic Areas: Química Odontología Nutrição Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: josep.guarro@urv.cat

Author identifier: 0000-0002-7839-7568

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000927

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Microbiology-Sgm. 166 (10): 936-946

APA: Castillo-Castañeda A; Cañas-Duarte SJ; Guevara-Suarez M; Guarro J; Restrepo S; Celis Ramírez AM (2020). Transcriptional response of fusarium oxysporum and neocosmospora solani challenged with amphotericin B or posaconazole. Microbiology-Sgm, 166(10), 936-946. DOI: 10.1099/mic.0.000927

Article's DOI: 10.1099/mic.0.000927

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications